Veronika Sanin and Christoph Heeß contributed equally to this work.
Recruitment of neural precursor cells from circumventricular organs of patients with cerebral ischaemia
Version of Record online: 9 JUL 2013
© 2012 The Authors. Neuropathology and Applied Neurobiology © 2012 British Neuropathological Society
Neuropathology and Applied Neurobiology
Volume 39, Issue 5, pages 510–518, August 2013
How to Cite
Sanin, V., Heeß, C., Kretzschmar, H. A. and Schüller, U. (2013), Recruitment of neural precursor cells from circumventricular organs of patients with cerebral ischaemia. Neuropathology and Applied Neurobiology, 39: 510–518. doi: 10.1111/j.1365-2990.2012.01301.x
- Issue online: 9 JUL 2013
- Version of Record online: 9 JUL 2013
- Accepted manuscript online: 17 SEP 2012 07:28AM EST
- Manuscript Accepted: 4 SEP 2012
- Manuscript Received: 12 MAR 2012
- Deutsche Krebshilfe (Max-Eder-Nachwuchsgruppenprogramm)
- circumventricular organs;
- stem cells;
Adult neurogenesis is well described in the subventricular zone of the lateral ventricle walls and in the subgranular zone of the hippocampal dentate gyrus. However, recent studies indicate that self-renewal of neural stem cells (NSCs) is not restricted to these niches, but that diverse areas of the adult brain are capable of generating new neurones and responding to various pathological alterations. In particular, NSCs have been identified in circumventricular organs (CVOs) of the adult mouse brain.
In order to detect possible neural stem or progenitor cells in CVOs of the human brain, we analysed post mortem human brain tissue from patients without neuropathological changes (n = 16) and brains from patients with ischaemic stroke (n = 16).
In all analysed CVOs (area postrema, median eminence, pineal gland and neurohypophysis) we observed cells with expression of early NSC markers, such as GFAP, nestin, vimentin, OLIG2 and PSA-NCAM, with some of them coexpressing Ki67 as a marker of cell proliferation. Importantly, stroke patients displayed an up to fivefold increase with respect to the relative number of Ki67- and OLIG2-expressing cells within their CVOs.
Our findings are compatible with a scenario where CVOs may serve as a further source of NSCs in the adult human brain and may contribute to neurogenesis and brain plasticity in the context of brain injury.