An immunocytochemical test for the diagnosis of antibodies to Plasmodium vivax

Authors

  • CARMEN BRACHO,

    1. Instituto Venezolano de Investigationes Cientificas, Centro de Microbiologia y Biologia Celular, Laboratorio de Inmunoparasitologia, Apartado 21827, Caracas 1020 A, Venezuela
    Search for more papers by this author
  • HILDA PEREZ

    Corresponding author
    1. Instituto Venezolano de Investigationes Cientificas, Centro de Microbiologia y Biologia Celular, Laboratorio de Inmunoparasitologia, Apartado 21827, Caracas 1020 A, Venezuela
    Search for more papers by this author

H. Perez

Abstract

Summary An immunocytochemical peroxidase test (ICPT) has been developed to allow serological measurement of the antibody response to Plasmodium vivax by light microscopy. Acetone fixed P. vivax erythrocyte stages were used as source of antigen. The immunofluorescent antibody test (IFAT) was used as reference test. In testing sera from individuals infected with P. vivax in southeastern Venezuela a high correlation (100%) was obtained between the ICPT and the IFAT. There were cross reactions with sera from patients with malaria by P. falciparum but not with those from patients with other parasitic diseases. Antibody titres as measured by the ICPT showed a positive correclation with past P. vivax malarial experiences.

Ancillary