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Antigenic protein modifications in Ehrlichia
Article first published online: 18 JAN 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
Volume 31, Issue 6, pages 296–303, June 2009
How to Cite
THOMAS, S., THIRUMALAPURA, N., CROSSLEY, E. C., ISMAIL, N. and WALKER, D. H. (2009), Antigenic protein modifications in Ehrlichia. Parasite Immunology, 31: 296–303. doi: 10.1111/j.1365-3024.2009.01099.x
- Issue published online: 26 MAY 2009
- Article first published online: 18 JAN 2009
- Received: 22 October 2008Accepted for publication: 9 January 2009
- Eastern blotting
To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE.