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Cold hardiness in the black rice bug, Scotinophara lurida

Authors

  • JUM RAE CHO,

    Corresponding author
    1. 1 Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon, Republic of Korea, 2Department of Agricultural Biology, College of Natural Sciences, Andong National University, Andong, Republic of Korea and 3Department of Entomology, School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
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  • 1 MINHO LEE,

    1. 1 Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon, Republic of Korea, 2Department of Agricultural Biology, College of Natural Sciences, Andong National University, Andong, Republic of Korea and 3Department of Entomology, School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
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  • 1 HONG SUN KIM,

    1. 1 Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon, Republic of Korea, 2Department of Agricultural Biology, College of Natural Sciences, Andong National University, Andong, Republic of Korea and 3Department of Entomology, School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
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  • 1 YOUNGGYUN KIM,

    1. 1 Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon, Republic of Korea, 2Department of Agricultural Biology, College of Natural Sciences, Andong National University, Andong, Republic of Korea and 3Department of Entomology, School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
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  • and 2 KYUNG SAENG BOO 3

    1. 1 Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon, Republic of Korea, 2Department of Agricultural Biology, College of Natural Sciences, Andong National University, Andong, Republic of Korea and 3Department of Entomology, School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
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Jum Rae Cho. Applied Entomology Division, Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea. Tel.: +82 31 290-0451; fax: +82 31 290 0407; e-mail: jrcho82@rda.go.kr

Abstract

Abstract The mechanisms and strategies for winter survival of the black rice bug Scotinophara lurida are investigated along with the relationship between cold hardiness and diapause. The ability of S. lurida to survive subzero temperatures varies depending on developmental stage, temperature and exposure duration. Mean supercooling point (SCP) varies from –7.6 to –10.7 °C with developmental stage, but is not significantly different between stages examined. The SCP also varies with season, being lowest in January and increasing rapidly in February and remaining almost at the same level (–7.3 to 9.6 °C) until April The osmolality of haemolymph of field-collected S. lurida adults rises dramatically from 53.9 mOsm kg−1 in August to 75.3 mOsm kg−1 in December, and then declines linearly to 57.0 mOsm kg−1 in May. Field-collected S. lurida adults show a peak glucose content in October, glycerol content in November and trehalose content in December. Only trehalose content decreases after the application of the juvenile hormone analogue, fenoxycarb, suggesting that trehalose is a cryoprotectant during diapause. These various physiological and biochemical traits related to cold tolerance in S. lurida may be, at least in part, under the control of juvenile hormone through the reproductive diapause programme.

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