Abstract The effect of photoperiod on pupariation time and the ability to secrete ecdysteroids by the larval ring gland of the flesh fly, Boettcherisca peregrina, are compared among three tropical non-diapausing colonies, Bp (forest), Bp (Luwuk) and Bp (NC) and one temperate diapausing colony of Bp (Tokyo). Non-diapausing colonies do not enter diapause under any photoperiod at 20°C, whereas the Bp (Tokyo) diapausing colony enters diapause at the pupal stage under a short-day photoperiod. The times required for 50% pupariation of Bp (forest), Bp (Luwuk) and Bp (Tokyo) are 34 h, 36 h 20 min and 34 h 40 min under short days (SD), respectively. However, Bp (NC) pupariates in a much shorter time (26 h 10 min) compared with the others. The 50% pupariation times of Bp (NC), Bp (forest) and Bp (Tokyo) under long days (LD) are 27 h, 25 h 50 min and 26 h 40 min, respectively. However, it is much longer in Bp (Luwuk) at 53 h 20 min. The rates of secretion of ecdysteroids by the larval ring glands in vitro in Bp (Tokyo) and Bp (forest) are six- to ten-fold higher under SD (6.00 and 3.69 ng) than under LD (0.60 ng), whereas the ring glands of Bp (Luwuk) and Bp (NC) secrete 3.06 and 8.25 ng ecdysteroids under LD and 5.18 and 10.53 ng under SD, respectively. These results suggest there are at least three different physiological types among non-diapausing colonies of B.peregrina: Bp (forest), Bp (Luwuk) and Bp (NC).