Plant cuticles sorb lipophilic compounds during enzymatic isolation


Jörg Schönherr, Lehrstuhl für Botanik, Technische Universität München, Arcisstrasse 21, D-8000 Munchen 2, FRG.


Abstract Plant cuticles sorb large amounts of hexadecanoic acid, octadecanoic acid and other lipophilic compounds (not identified) when incubated in cell slurries obtained by enzymatically digesting leaves or fruits. These extraneous substances cannot be removed completely and selectively after cuticle isolation, nor is it possible to prevent sorption by optimizing isolation procedures. It is, therefore, impossible to estimate amounts and composition of intracuticular soluble lipids using enzymatically isolated cuticles, as has been done in the past. Extraneous substances sorbed during isolation do not affect water permeability of the cuticles.