SEARCH

SEARCH BY CITATION

Keywords:

  • Lycopersicon esculentum Mill. cv. T-5;
  • tomato;
  • ammonium;
  • isotope discrimination;
  • nitrate;
  • nitrogen isotopes

ABSTRACT

Studies that quantify plant δ15N often assume that fractionation during nitrogen uptake and intra-plant variation in δ15N are minimal. We tested both assumptions by growing tomato (Lycopersicon esculetum Mill. cv. T-5) at NH4+ or NO3 concentrations typical of those found in the soil. Fractionation did not occur with uptake; whole-plant δ15N was not significantly different from source δ15 N for plants grown on either nitrogen form. No intra-plant variation in δ15N was observed for plants grown with NH+4. In contrast. δ15N of leaves was as much as 5.8% greater than that of roots for plants grown with NO3. The contrasting patterns of intra-plant variation are probably caused by different assimilation patterns. NH+4 is assimilated immediately in the root, so organic nitrogen in the shoot and root is the product of a single assimilation event. NO3 assimilation can occur in shoots and roots. Fractionation during assimilation caused the δ15N of NO3 to become enriched relative to organic nitrogen; the δ15N of NO3 was 11.1 and 12.9% greater than the δ15N of organic nitrogen in leaves and roots, respectively. Leaf δ15N may therefore be greater than that of roots because the NO3 available for assimilation in leaves originates from a NO3 pool that was previously exposed to nitrate assimilation in the root.