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Complementation of notabilis, an abscisic acid-deficient mutant of tomato: importance of sequence context and utility of partial complementation

  1. A. J. THOMPSON1,
  2. E. T. THORNE2,†,
  3. A. BURBIDGE3,
  4. A. C. JACKSON1,
  5. R. E. SHARP2,
  6. I. B. TAYLOR3

Article first published online: 9 FEB 2004

DOI: 10.1111/j.1365-3040.2003.01164.x

Issue

Plant, Cell & Environment

Plant, Cell & Environment

Volume 27, Issue 4, pages 459–471, April 2004

Additional Information

How to Cite

THOMPSON, A. J., THORNE, E. T., BURBIDGE, A., JACKSON, A. C., SHARP, R. E. and TAYLOR, I. B. (2004), Complementation of notabilis, an abscisic acid-deficient mutant of tomato: importance of sequence context and utility of partial complementation. Plant, Cell & Environment, 27: 459–471. doi: 10.1111/j.1365-3040.2003.01164.x

Author Information

  1. 1

    Crop Biotechnology and Agronomy, Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, UK,

  2. 2

    Department of Agronomy, Plant Sciences Unit, 1–87 Agriculture Building, University of Missouri, Columbia, Missouri, 65211, USA and

  3. 3

    Plant Sciences Division, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, UK

  1. †

    *Current address: Viticulture and Enology Department, University of California, Davis, CA 95616, USA.

*Andrew Thompson. Fax: +44 1789470552; e-mail: andrew.thompson@hri.ac.uk

  1. †

    *Current address: Viticulture and Enology Department, University of California, Davis, CA 95616, USA.

Publication History

  1. Issue published online: 1 MAR 2004
  2. Article first published online: 9 FEB 2004
  3. Received 16 June 2003; received in revised form 6 November 2003; accepted for publication 24 November 2003

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Keywords:

  • 9-cis-epoxycarotenoid dioxygenase;
  • growth;
  • matrix attachment region;
  • promoter;
  • tomato;
  • water stress

Abstract

The abscisic acid (ABA)-deficient tomato mutant notabilis (not) is believed to be a null mutation in the gene LeNCED1, encoding a 9-cis-epoxycarotenoid dioxygenase involved in ABA biosynthesis. We have sequenced and analysed a 19 kb genomic clone containing LeNCED1 and 5.4 kb of its promoter. This clone was transferred to not homozygotes and several non-wilty transformed plants were obtained. The basal ABA content, water relations, shoot and root growth, adventitious rooting, ethylene evolution and ability to accumulate ABA under water stress are described for two of these lines, notcomp.13 and notcomp.1. Partial complementation was observed for most parameters measured for notcomp.1. Full complementation was observed in notcomp.13 for all parameters measured in whole plants under well-watered and water-stressed conditions. These data provide further evidence that LeNCED1 is the wild-type allele of the not mutant gene. However, notcomp.13 was unable to accumulate the wild-type levels of ABA in rapidly dehydrated leaves, indicating that it too was only partially complemented. Since LeNCED1 is an environmentally regulated gene encoding a rate-limiting enzyme, precise levels and patterns of gene expression may be needed to fully recreate wild-type phenotype. The utility of partially complemented lines to study the role of ABA in plant responses to stress conditions, and in promoter analysis, is discussed.

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