A portable integrated automatic apparatus for the real-time monitoring of bioluminescence in plants

Authors

  • KAZUHISA OKAMOTO,

    1. Center for Gene Research, Nagoya University, Furo, Chikusa, Nagoya 464–8602, Japan,
    2. Aichi Science and Technology Foundation, 2-4-7 Marunouchi, Naka, Nagoya 460–0002, Japan,
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    • *

      These two authors contributed equally to this work.

  • KIYOSHI ONAI,

    1. Center for Gene Research, Nagoya University, Furo, Chikusa, Nagoya 464–8602, Japan,
    2. Bio-oriented Technology Research Advancement Institution (BRAIN), Nagoya University, Furo, Chikusa, Nagoya 464–8602, Japan,
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    • *

      These two authors contributed equally to this work.

  • TAKAYOSHI FURUSAWA,

    1. R & D Group, Bioscience Department, Aloka Co., LTD., 6-22-1, Mure, Mitaka, Tokyo 181–8622, Japan and
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  • MASAHIRO ISHIURA

    Corresponding author
    1. Center for Gene Research, Nagoya University, Furo, Chikusa, Nagoya 464–8602, Japan,
    2. Aichi Science and Technology Foundation, 2-4-7 Marunouchi, Naka, Nagoya 460–0002, Japan,
    3. Bio-oriented Technology Research Advancement Institution (BRAIN), Nagoya University, Furo, Chikusa, Nagoya 464–8602, Japan,
    4. Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464–8602, Japan
      Masahiro Ishiura. Fax: + 81 52 789 4526; e-mail: ishiura@gene.nagoya-u.ac.jp
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Masahiro Ishiura. Fax: + 81 52 789 4526; e-mail: ishiura@gene.nagoya-u.ac.jp

ABSTRACT

Real-time monitoring of gene expression by a bioluminescence reporter gene is a powerful method for large-scale, detailed analysis of gene expression in living cells and large-scale screening of mutants. We have developed a portable, compact, integrated automatic bioluminescence-monitoring apparatus that can continuously monitor 960 individual plant seedlings or micro-organism colonies under uniform light conditions at temperatures up to 50 °C. The apparatus gave reproducible and reliable results for both bioluminescence photon counts and period length of bioluminescence rhythms of Arabidopsis reporter strain. Using the apparatus, we measured bioluminescence rhythms in the thermophilic cyanobacterium Thermosynechococcus at temperature up to 43 °C. We also monitored the expression of the flowering regulator gene CONSTANS in Arabidopsis as bioluminescence in high time resolution under different photoperiodic conditions. The high-throughput bioluminescence-monitoring apparatus developed here is a powerful tool for real-time monitoring of gene expression and gene function.

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