Present address: Institute for Plant Biotechnology, Natural Sciences Building Department of Genetics, Faculty of AgriSciences, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa.
Genetic variation in pea (Pisum sativum L.) demonstrates the importance of root but not shoot C/N ratios in the control of plant morphology and reveals a unique relationship between shoot length and nodulation intensity
Article first published online: 19 JUL 2007
Plant, Cell & Environment
Volume 30, Issue 10, pages 1256–1268, October 2007
How to Cite
LUDIDI, N. N., PELLNY, T. K., KIDDLE, G., DUTILLEUL, C., GROTEN, K., VAN HEERDEN, P. D. R., DUTT, S., POWERS, S. J., RÖMER, P. and FOYER, C. H. (2007), Genetic variation in pea (Pisum sativum L.) demonstrates the importance of root but not shoot C/N ratios in the control of plant morphology and reveals a unique relationship between shoot length and nodulation intensity. Plant, Cell & Environment, 30: 1256–1268. doi: 10.1111/j.1365-3040.2007.01699.x
- Issue published online: 19 JUL 2007
- Article first published online: 19 JUL 2007
- Received 22 March 2007; received in revised form 31 May 2007; accepted for publication 5 June 2007
- carbon/nitrogen interactions;
- grain legumes;
- natural genetic variation;
- shoot/root ratios;
- stem length
Nodule numbers are regulated through systemic auto-regulatory signals produced by shoots and roots. The relative effects of shoot and root genotype on nodule numbers together with relationships to organ biomass, carbon (C) and nitrogen (N) status, and related parameters were measured in pea (Pisum sativum) exploiting natural genetic variation in maturity and apparent nodulation intensity. Reciprocal grafting experiments between the early (Athos), intermediate (Phönix) and late (S00182) maturity phenotypes were performed and Pearson's correlation coefficients for the parameters were calculated. No significant correlations were found between shoot C/N ratios and plant morphology parameters, but the root C/N ratio showed a strong correlation with root fresh and dry weights as well as with shoot fresh weight with less significant interactions with leaf number. Hence, the root C/N ratio rather than shoot C/N had a predominant influence on plant morphology when pea plants are grown under conditions of symbiotic nitrogen supply. The only phenotypic characteristic that showed a statistically significant correlation with nodulation intensity was shoot length, which accounted for 68.5% of the variation. A strong linear relationship was demonstrated between shoot length and nodule numbers. Hence, pea nodule numbers are controlled by factors related to shoot extension, but not by shoot or root biomass accumulation, total C or total N. The relationship between shoot length and nodule numbers persisted under field conditions. These results suggest that stem height could be used as a breeding marker for the selection of pea cultivars with high nodule numbers and high seed N contents.