Molecular cloning, characterization and regulation of two different NADH-glutamate synthase cDNAs in bean nodules
Version of Record online: 23 JAN 2008
© 2008 The Authors
Plant, Cell & Environment
Volume 31, Issue 4, pages 454–472, April 2008
How to Cite
BLANCO, L., REDDY, P. M., SILVENTE, S., BUCCIARELLI, B., KHANDUAL, S., ALVARADO-AFFANTRANGER, X., SÁNCHEZ, F., MILLER, S., VANCE, C. and LARA-FLORES, M. (2008), Molecular cloning, characterization and regulation of two different NADH-glutamate synthase cDNAs in bean nodules. Plant, Cell & Environment, 31: 454–472. doi: 10.1111/j.1365-3040.2008.01774.x
- Issue online: 23 JAN 2008
- Version of Record online: 23 JAN 2008
- Received 25 August 2007; received in revised form 13 December 2007; accepted for publication 19 December 2007
Figure S1. Sequences of the Phaseolus vulgaris NADH-dependent glutamate synthase (NADH-GOGAT)-I and NADH-GOGAT-II 5′ flanking regions. The upstream 1501 bp and 1366 bp flanking regions of the NADH-GOGAT-I and NADH-GOGAT-II genes, respectively, from the putative translation start codons are shown. TATA box is highlighted in red, and CAAT boxes in green, and predicted transcription start site is shaded in blue. Uppercase letters denoted in black correspond to the 1065 bp and 1174 bp regions of the putative NADH-GOGAT-I and NADH-GOGAT-II promoters, whereas the letters indicated in blue correspond to the 436 bp and 192 bp 5′ untranslated regions of the respective cDNAs. The first nucleotide upstream of the NADH-GOGAT-I and NADH-GOGAT-II ORFs is labelled as −1.
Table S1. Profiles of PCR cycles employed for isolating full-length PvNADH-dependent glutamate synthase (NADH-GOGAT)-I and -II cDNAs, and RT-PCR
Table S2. Primers used for obtaining the 5′ regions of PvNADH-dependent glutamate synthase (NADH-GOGAT)-I and -II full-length cDNAs, and RT-PCR analysis.
Table S3. PCR primers used for genome walking of the 5′ flanking region and amplification of the full-length promoters of PvNADH-dependent glutamate synthas e (NADH-GOGAT)-I and -II genes.
|PCE_1774_sm_Figure_S1_and_Tables_S1-S3.pdf||69K||Supporting info item|
|PCE_figure_s1_and_tables_s1-s3.pdf||69K||Supporting info item|