Tomato fruit ascorbic acid content is linked with monodehydroascorbate reductase activity and tolerance to chilling stress

Authors

  • R. STEVENS,

    Corresponding author
    1. INRA, UR1052, Unité de génétique et amélioration des fruits et légumes, Domaine St Maurice BP94, 84143 Montfavet,
      R. Stevens. Fax: +33 (0)4 32 72 27 02; e-mail: stevens@avignon.inra.fr
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    • *

      These authors contributed equally to the paper.

  • D. PAGE,

    1. INRA, UMR A408, Sécurité et qualité des produits d'origine végétale, Domaine St Paul, Site Agroparc, 84914 Avignon, France and
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    • *

      These authors contributed equally to the paper.

  • B. GOUBLE,

    1. INRA, UMR A408, Sécurité et qualité des produits d'origine végétale, Domaine St Paul, Site Agroparc, 84914 Avignon, France and
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  • C. GARCHERY,

    1. INRA, UR1052, Unité de génétique et amélioration des fruits et légumes, Domaine St Maurice BP94, 84143 Montfavet,
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  • D. ZAMIR,

    1. Hebrew University of Jerusalem, Faculty of Agriculture, Department of Field, Vegetable Crops and Genetics, Israel
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  • M. CAUSSE

    1. INRA, UR1052, Unité de génétique et amélioration des fruits et légumes, Domaine St Maurice BP94, 84143 Montfavet,
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R. Stevens. Fax: +33 (0)4 32 72 27 02; e-mail: stevens@avignon.inra.fr

ABSTRACT

Quantitative trait loci (QTL) mapping is a step towards the identification of factors regulating traits such as fruit ascorbic acid content. A previously identified QTL controlling variations in tomato fruit ascorbic acid has been fine mapped and reveals that the QTL has a polygenic and epistatic architecture. A monodehydroascorbate reductase (MDHAR) allele is a candidate for a proportion of the increase in fruit ascorbic acid content. The MDHAR enzyme is active in different stages of fruit ripening, shows increased activity in the introgression lines containing the wild-type (Solanum pennellii) allele, and responds to chilling injury in tomato along with the reduced/oxidized ascorbate ratio. Low temperature storage of different tomato introgression lines with all or part of the QTL for ascorbic acid and with or without the wild MDHAR allele shows that enzyme activity explains 84% of the variation in the reduced ascorbic acid levels of tomato fruit following storage at 4 °C, compared with 38% at harvest under non-stress conditions. A role is indicated for MDHAR in the maintenance of ascorbate levels in fruit under stress conditions. Furthermore, an increased fruit MDHAR activity and a lower oxidation level of the fruit ascorbate pool are correlated with decreased loss of firmness because of chilling injury.

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