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Complex phenotypic profiles leading to ozone sensitivity in Arabidopsis thaliana mutants

  1. KIRK OVERMYER1,†,*,
  2. HANNES KOLLIST1,2,†,
  3. HANNELE TUOMINEN1,‡,
  4. CHRISTIAN BETZ3,
  5. CHRISTIAN LANGEBARTELS3,
  6. GUNNAR WINGSLE4,
  7. SAIJALIISA KANGASJÄRVI5,
  8. GÜNTER BRADER6,
  9. PHIL MULLINEAUX7,§,
  10. JAAKKO KANGASJÄRVI1

Article first published online: 2 JUN 2008

DOI: 10.1111/j.1365-3040.2008.01837.x

Issue

Plant, Cell & Environment

Plant, Cell & Environment

Volume 31, Issue 9, pages 1237–1249, September 2008

Additional Information

How to Cite

OVERMYER, K., KOLLIST, H., TUOMINEN, H., BETZ, C., LANGEBARTELS, C., WINGSLE, G., KANGASJÄRVI, S., BRADER, G., MULLINEAUX, P. and KANGASJÄRVI, J. (2008), Complex phenotypic profiles leading to ozone sensitivity in Arabidopsis thaliana mutants. Plant, Cell & Environment, 31: 1237–1249. doi: 10.1111/j.1365-3040.2008.01837.x

Author Information

  1. 1

    Plant Biology, Department of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland;

  2. 2

    Institute of Technology, University of Tartu, Tartu 50411, Estonia;

  3. 3

    Institute of Biochemical Plant Pathology, Helmholtz Center München, D-85764 Oberschleissheim, Germany;

  4. 4

    Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-90187 Umeå, Sweden;

  5. 5

    Plant Physiology and Molecular Biology, Department of Biology, University of Turku, FI-20014 Turku, Finland;

  6. 6

    Genetics, Department of Biological and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, Finland; and

  7. 7

    Department of Disease and Stress Biology, John Innes Centre, Colney, Norwich, NR4 7UH, United Kingdom

  1. †

    These authors contributed equally to this study.

  2. ‡

    Current address: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, S-90187 Umeå, Sweden

  3. §

    Current address: Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK

*K. Overmyer. Fax: +358-9-19159552; e-mail: kirk.overmyer@helsinki.fi

  • †

    These authors contributed equally to this study.

  • ‡

    Current address: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, S-90187 Umeå, Sweden

  • §

    Current address: Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK

Publication History

  1. Issue published online: 13 AUG 2008
  2. Article first published online: 2 JUN 2008
  3. Received 22 December 2007; received in revised form 30 April 2008; accepted for publication 1 May 2008

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Keywords:

  • reticulata;
  • chloroplast;
  • genetics;
  • leaf organogenesis;
  • oxidative stress;
  • reactive oxygen species;
  • stomata

ABSTRACT

Genetically tractable model plants offer the possibility of defining the plant O3 response at the molecular level. To this end, we have isolated a collection of ozone (O3)-sensitive mutants of Arabidopsis thaliana. Mutant phenotypes and genetics were characterized. Additionally, parameters associated with O3 sensitivity were analysed, including stomatal conductance, sensitivity to and accumulation of reactive oxygen species, antioxidants, stress gene-expression and the accumulation of stress hormones. Each mutant has a unique phenotypic profile, with O3 sensitivity caused by a unique set of alterations in these systems. O3 sensitivity in these mutants is not caused by gross deficiencies in the antioxidant pathways tested here. The rcd3 mutant exhibits misregulated stomata. All mutants exhibited changes in stress hormones consistent with the known hormonal roles in defence and cell death regulation. One mutant, dubbed re-8, is an allele of the classic leaf development mutant reticulata and exhibits phenotypes dependent on light conditions. This study shows that O3 sensitivity can be determined by deficiencies in multiple interacting plant systems and provides genetic evidence linking these systems.

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