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Figure S1. Diagrams of RNAi constructs and their expression in transgenic roots. Diagrams (not drawn to scale) representing the PvPHR1-RNAi (a) and PvDCL1-RNAi (c) gene construct Gateway cassettes, cloned in the pTDT-DC-RNAi vector. The constructs consist of inverted repeats of a PvPHR1 or PvDCL1 gene fragments, separated by the WRKY intron and driven by the CaMV 35S promoter. Arrows indicate the position of the primers used to verify the orientation of the inverted repeats by PCR amplification. Expression of PvPHR1-RNAi (b) and PvDCL1-RNAi (d) were determined by sRT-PCR from putative transgenic roots as compared with control (empty vector) roots from composite plants grown in +P and −P conditions. The TDT and actin genes were used as internal controls.

Figure S2. Nucleotide sequence of PvmiR399 and Pv4 and deduced amino acid sequences of PvDCL1 and PvPHO2 and as compared with Arabidopsis orthologous genes. (a) Comparison between members of AtmiR399 gene family and homologous PvmiR399 nucleotide sequences. Conserved bases are highlighted in black. (b) Comparison of deduced amino acid sequence of AtDCL1 and PvDCL1; conserved regions corresponding to double-strand RNA-binding domain are highlighted. (c) Alignment of AtmiR399 and PvmiR399 with complementary sequences from At4 and Pv4, respectively. Mispaired nucleotides are shown in red. (d) Comparison of deduced amino acid sequence of AtPHO2 and PvPHO2; conserved ubiquitin-conjugating enzyme E2 catalytic domain (UBCc) is highlighted.

Table S1. Selected P-responsive common bean genes: annotation, designed primers and sRT-PCR conditions used for expression analysis.

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PCE_1883_sm_Table_S1.doc41KSupporting info item
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PCE_1883_sm_Fig_S2.tif802KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.