Essential role of MYB transcription factor: PvPHR1 and microRNA: PvmiR399 in phosphorus-deficiency signalling in common bean roots
Article first published online: 2 SEP 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
Plant, Cell & Environment
Volume 31, Issue 12, pages 1834–1843, December 2008
How to Cite
VALDÉS-LÓPEZ, O., ARENAS-HUERTERO, C., RAMÍREZ, M., GIRARD, L., SÁNCHEZ, F., VANCE, C. P., LUIS REYES, J. and HERNÁNDEZ, G. (2008), Essential role of MYB transcription factor: PvPHR1 and microRNA: PvmiR399 in phosphorus-deficiency signalling in common bean roots. Plant, Cell & Environment, 31: 1834–1843. doi: 10.1111/j.1365-3040.2008.01883.x
- Issue published online: 11 NOV 2008
- Article first published online: 2 SEP 2008
- Received 18 June 2008; received in revised form 28 August 2008; accepted for publication 28 August 2008
Figure S1. Diagrams of RNAi constructs and their expression in transgenic roots. Diagrams (not drawn to scale) representing the PvPHR1-RNAi (a) and PvDCL1-RNAi (c) gene construct Gateway cassettes, cloned in the pTDT-DC-RNAi vector. The constructs consist of inverted repeats of a PvPHR1 or PvDCL1 gene fragments, separated by the WRKY intron and driven by the CaMV 35S promoter. Arrows indicate the position of the primers used to verify the orientation of the inverted repeats by PCR amplification. Expression of PvPHR1-RNAi (b) and PvDCL1-RNAi (d) were determined by sRT-PCR from putative transgenic roots as compared with control (empty vector) roots from composite plants grown in +P and −P conditions. The TDT and actin genes were used as internal controls.
Figure S2. Nucleotide sequence of PvmiR399 and Pv4 and deduced amino acid sequences of PvDCL1 and PvPHO2 and as compared with Arabidopsis orthologous genes. (a) Comparison between members of AtmiR399 gene family and homologous PvmiR399 nucleotide sequences. Conserved bases are highlighted in black. (b) Comparison of deduced amino acid sequence of AtDCL1 and PvDCL1; conserved regions corresponding to double-strand RNA-binding domain are highlighted. (c) Alignment of AtmiR399 and PvmiR399 with complementary sequences from At4 and Pv4, respectively. Mispaired nucleotides are shown in red. (d) Comparison of deduced amino acid sequence of AtPHO2 and PvPHO2; conserved ubiquitin-conjugating enzyme E2 catalytic domain (UBCc) is highlighted.
Table S1. Selected P-responsive common bean genes: annotation, designed primers and sRT-PCR conditions used for expression analysis.
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