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Figure S1. Enzyme activities calculated on a fresh weight basis. Enzyme activities were measured at the end of the light period (open bars) and end of the night period (solid bars) in wild-type Arabidopsis growing in a 12/12, 8/16, 4/20, 3/21 or 2/22 h light/dark cycle. (a) Ribulose 1·5-bisphosphate carboxylase/oxygenase (Rubisco). (b) Phosphoglycerate kinase. (c) NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (NADP-GAPDH). (d) Fructose-bisphosphate (FBP)-aldolase. (e) Transketolase. (f) Plastid phosphoglucose isomerase (g) Phosphoglucomutase. (h) ADP-glucose pyrophosphorylase (AGPase). (i) Cytosolic phosphoglucose isomerase. (j) Sucrose phosphate synthase. (k) Acid invertase. (l) Pyrophosphate-dependent phosphofructokinase. (m) ATP-dependent phosphofructokinase. (n) Pyruvate kinase. (o) Phosphoenolpyruvate (PEP) carboxylase. (p) Citrate synthase. (q) NAD-dependent isocitrate dehydrogenase. (r) Fumarase. (s) NAD-dependent malate dehydrogenase. (t) Nitrate reductase. (u) Glutamine synthetase. (v) Ferredoxin-dependent glutamate synthase (GOGAT). (w) Glutamate dehydrogenase (glutamate DH). The results are the mean ± SE. (n = 5 separate samples).

Figure S2. Comparison of the changes of enzyme activity in pgm and in wild-type Col-0 after 7 d of extended darkness. The plot is recalculated from data in Gibon et al. (2004b).

Figure S3. Comparison of the activity of enzymes from the Calvin cycle, glycolysis, the tricarboxylic acid cycle, sucrose and starch synthesis and nitrogen metabolism in short photoperiods, in prolonged darkness and in the starchless pgm mutant. Rubisco, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (NADP-GAPDH), transketolase, AGPase, sucrose phosphate synthase, pyrophosphate (PPi)-dependent phosphofructokinase, ATP-dependent phosphofructokinase, pyruvate kinase, phosphoenolpyruvate (PEP) carboxylase, fumarase, ferredoxin-dependent glutamate synthase, glutamine synthetase and nitrate reductase activities were measured in wild-type Col-0 rosettes at the end of the light period (EL, white sectors) and the end of the dark period (EN, grey sectors) in material harvested from plants 17 d after transfer to a 12/12, 8/16, 4/20, 3/21 or 2/22 h light/h dark photoregime. For comparison, the same enzyme activities are shown for wild-type Col-0 grown in a 12 h light/12 h dark cycle before transfer to continuous darkness for 2, 3 or 7 d, and in the starchless pgm mutant grown in a 12 h light/12 h dark regime and harvested at the end of the day (EL, white sectors) and night (EN, grey sectors). The results are the mean ± SE of five replicate samples.

Table S1. Metabolite levels and enzyme activities in wild-type Col-0 grown in a 12 h light/12 h dark, a 8 h light/16 h dark, a 4 h light/20 h dark, a 3 h light/21 h dark and a 2 h light/22 h dark photoperiod, in the starchless pgm mutant grown in a 12 h light/12 h dark photoperiod, or in wild-type Col-0 subjected to a 48, 72 or 148 h extension of the night. The data are provided as means ± SE of five replicate samples.

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