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The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1/MYB12 gene in response to light and ultraviolet-B radiation

  1. RALF STRACKE1,†,
  2. JEAN-JACQUES FAVORY2,†,
  3. HENRIETTE GRUBER2,†,
  4. LUTZ BARTELNIEWOEHNER1,
  5. SEBASTIAN BARTELS2,
  6. MELANIE BINKERT2,
  7. MARKUS FUNK2,
  8. BERND WEISSHAAR1,
  9. ROMAN ULM2,3,*

Article first published online: 4 NOV 2009

DOI: 10.1111/j.1365-3040.2009.02061.x

Issue

Plant, Cell & Environment

Plant, Cell & Environment

Volume 33, Issue 1, pages 88–103, January 2010

Additional Information

How to Cite

STRACKE, R., FAVORY, J.-J., GRUBER, H., BARTELNIEWOEHNER, L., BARTELS, S., BINKERT, M., FUNK, M., WEISSHAAR, B. and ULM, R. (2010), The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1/MYB12 gene in response to light and ultraviolet-B radiation. Plant, Cell & Environment, 33: 88–103. doi: 10.1111/j.1365-3040.2009.02061.x

Author Information

  1. 1

    Department of Biology, Chair of Genome Research, Bielefeld University, D-33594 Bielefeld, Germany,

  2. 2

    Faculty of Biology, Institute of Biology II, University of Freiburg, D-79104 Freiburg, Germany and

  3. 3

    Centre for Biological Signalling Studies (bioss), University of Freiburg, D-79104 Freiburg, Germany

  1. †

    These authors contributed equally to this work.

*R. Ulm. Fax: +49 761 2032612; e-mail: roman.ulm@biologie.uni-freiburg.de

  • †

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 10 DEC 2009
  2. Article first published online: 4 NOV 2009
  3. Received 11 August 2009; received in revised form 12 October 2009; accepted for publication 13 October 2009

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Keywords:

  • abiotic stress;
  • flavonoids;
  • gene expression;
  • MYB12;
  • UV-B tolerance

ABSTRACT

Plants fend off potentially damaging ultraviolet (UV)-B radiation by synthesizing and accumulating UV-B-absorbing flavonols that function as sunscreens. Regulation of this biosynthetic pathway is largely transcriptional and controlled by a network of transcription factors, among which the PRODUCTION OF FLAVONOL GLYCOSIDES (PFG) family of R2R3-MYB transcription factors was recently identified with a pivotal function. Here, we describe the response of Arabidopsis seedlings to narrow-band UV-B radiation at the level of phenylpropanoid pathway genes using whole-genome transcriptional profiling and identify the corresponding flavonol glycosides accumulating under UV-B. We further show that the bZIP transcriptional regulator ELONGATED HYPOCOTYL5 (HY5) is required for the transcriptional activation of the PFG1/MYB12 and PFG3/MYB111 genes under UV-B and visible light. A synthetic protein composed of HY5 with the VP16 activation domain is sufficient to activate PFG1/MYB12 expression in planta. However, even though myb11 myb12 myb111 triple mutants have strongly reduced CHS levels in darkness as well as in constant light, neither light- nor UV-B-inducibility seems impaired. Notwithstanding this, absence of the three PFG family transcription factors results in reduced UV-B tolerance, whereas PFG1/MYB12 overexpression leads to an increased tolerance. Thus, our data suggest that HY5-dependent regulation of PFG gene expression contributes to the establishment of UV-B tolerance.

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