Intraspecific variation in the Populus balsamifera drought transcriptome

Authors

  • ERIN T. HAMANISHI,

    1. Faculty of Forestry, University of Toronto, 33 Willcocks St., Toronto, ON M5S 3B3, Canada
    2. Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada
    Search for more papers by this author
  • SHEROSHA RAJ,

    1. Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada
    2. Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada
    Search for more papers by this author
  • OLIVIA WILKINS,

    1. Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada
    2. Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada
    Search for more papers by this author
  • BARB R. THOMAS,

    1. Alberta-Pacific Forest Industries Inc. P.O. Box 8000 Boyle, Alberta, T0A 0M0, Canada
    2. Department of Renewable Resources, University of Alberta, 731 General Services Building, Edmonton, AB T6G 2H1, Canada
    Search for more papers by this author
  • SHAWN D. MANSFIELD,

    1. Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC V6T 1Z4, Canada
    Search for more papers by this author
  • AINE L. PLANT,

    1. Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada
    Search for more papers by this author
  • MALCOLM M. CAMPBELL

    Corresponding author
    1. Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada
    2. Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada
    Search for more papers by this author

  • Contributions:
    ETH, SDM, ALP and MMC designed research; ETH, SR, BT, SDM, ALP and MMC organised experimental logistics including transfer and establishment of biological materials; ETH, SR, and OW performed research; ETH, OW, and MMC analysed data; ETH and MMC wrote manuscript with editorial assistance from SR, OW, BT, SDM, ALP and MMC.

M. M. Campbell. Fax: +1 416 978 5878; e-mail: malcolm.campbell@utoronto.ca

ABSTRACT

Drought is a major limitation to the growth and productivity of trees in the ecologically and economically important genus Populus. The ability of Populus trees to contend with drought is a function of genome responsiveness to this environmental insult, involving reconfiguration of the transcriptome to appropriately remodel growth, development and metabolism. Here we test hypotheses aimed at examining the extent of intraspecific variation in the drought transcriptome using six different Populus balsamifera L. genotypes and Affymetrix GeneChip technology. Within a given genotype there was a positive correlation between the magnitude of water-deficit induced changes in transcript abundance across the transcriptome, and the capacity of that genotype to maintain growth following water deficit. Genotypes that had more similar drought-responsive transcriptomes also had fewer genotypic differences, as determined by microarray-derived single feature polymorphism (SFP) analysis, suggesting that responses may be conserved across individuals that share a greater degree of genotypic similarity. This work highlights the fact that a core species-level response can be defined; however, the underpinning genotype-derived complexities of the drought response in Populus must be taken into consideration when defining both species- and genus-level responses.

Ancillary