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Supporting information Calculation of anabolic demand terms.

Table S1. Gross stoichiometries for biosynthesis of eight amino acids and their carbon skeleton precursors. Numbers shown are the number of molecules of the compound in the column heading that are consumed in the synthesis of one molecule of the compound named in the first column. Positive numbers represent net yield rather than consumption. Net stoichiometries are given in Table S2.

Table S2. Net stoichiometries for biosynthesis of eight amino acids, and the percent of total amino acids contributed by each in calculations of anabolic demand/supply terms.

Table S3. Net production of NADH, NADPH, ATP and CO2 per mole of source carbon in the biosynthesis and maintenance of five classes of compounds used to calculate anabolic demand in the current study. Dimensions are moles of NADH, NADPH, CO2 or ATP per mole of source carbon consumed in biosynthesis of products listed in the column headings. Two values are given for NADH and NADPH, based on nitrogen assimilation beginning from either nitrate or ammonium (N assimilation affects stoichiometries for phospholipid synthesis here because we used phosphatidyl serine as the archetype for phosphate-linked R group). Three values are given for ATP under the heading of protein. For biosynthesis, (1) represents de novo synthesis of amino acids only; and (2) represents both de novo synthesis and polymerisation of amino acids. For maintenance, (3) represents degradation of proteins and repolymerisation of existing amino acids only. Calculations are described above under ‘Supply/demand stoichiometries for biosynthesis and maintenance processes.’

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