Figure S1. TT19 co-expression with other flavonoid pathway genes in tt19 mutants. (a) A network of flavonoid genes co-expressed with the TT19 gene as determined by in silico analysis of microarray data in the ATTED-II database. Number values represent expression relative to TT19. (b) Q-PCR expression levels of co-expressed flavonoid genes from panel A in 10-day seedlings of tt19-1 and tt19-4 mutants relative to Col-4. (c) Gene expression profiles of non-co-expressed flavonoid genes in 10-day seedlings of tt19-1 and tt19-4 mutants. (b,c) Expression profiles represent means (±SE) from three independent experiments relative to WT Col-4, and significance (*) is indicated at P < 0.05).

Figure S2. Subcellular localization of TT19-WT and TT19-4 proteins. (a) Empty vector control; (b) WT-TT19-GFP; (c) TT19-4-GFP; (d) Co-introduction of an empty vector control with a cytoplasmic membrane red fluorescent marker; (e) Co-introduction of WT-TT19-GFP with a cytoplasmic membrane red fluorescent marker showing localization of TT19-GFP in cytoplasmic strands; (f) Co-introduction of TT19-4-GFP with a cytoplasmic membrane red fluorescent marker showing a similar localization pattern as wild-type TT19.

Table S1. Primers used in this study.

Table S2. List of flavonoids, proanthocyanidin dimers, anthocyanins, and phenolic acid derivatives tested as substrates in glutathionylation and binding reactions with a recombinant TT19 protein.

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