Down-regulation of SymRK correlates with a deficiency in vascular bundle development in Phaseolus vulgaris nodules

Authors

  • ROSANA SÁNCHEZ-LÓPEZ,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
    Search for more papers by this author
  • DAVID JÁUREGUI,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
    Search for more papers by this author
  • NOREIDE NAVA,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
    Search for more papers by this author
  • XÓCHITL ALVARADO-AFFANTRANGER,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
    Search for more papers by this author
  • JESÚS MONTIEL,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
    Search for more papers by this author
  • OLIVIA SANTANA,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
    Search for more papers by this author
  • FEDERICO SANCHEZ,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
    Search for more papers by this author
  • CARMEN QUINTO

    Corresponding author
    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
      C. Quinto. Fax: +52 777 313 6600; e-mail: quinto@ibt.unam.mx
    Search for more papers by this author

C. Quinto. Fax: +52 777 313 6600; e-mail: quinto@ibt.unam.mx

ABSTRACT

The symbiotic interaction of legumes and rhizobia results in the formation of nitrogen-fixing nodules. Nodulation depends on the finely coordinated expression of a battery of genes involved in the infection and the organogenesis processes. After Nod factor perception, symbiosis receptor kinase (SymRK) receptor triggers a signal transduction cascade essential for nodulation leading to cortical cell divisions, infection thread (IT) formation and final release of rhizobia to the intracellular space, forming the symbiosome. Herein, the participation of SymRK receptor during the nodule organogenesis in Phaseolus vulgaris is addressed. Our findings indicate that besides its expression in the nodule epidermis, in IT, and in uninfected cells of the infection zone, PvSymRK immunolocalizes in the root and nodule vascular system. On the other hand, knockdown expression of PvSymRK led to the formation of scarce and defective nodules, which presented alterations in both IT/symbiosome formation and vascular system.

Ancillary