Whole organ, venation and epidermal cell morphological variations are correlated in the leaves of Arabidopsis mutants
Article first published online: 22 SEP 2011
© 2011 Blackwell Publishing Ltd
Plant, Cell & Environment
Volume 34, Issue 12, pages 2200–2211, December 2011
How to Cite
PÉREZ-PÉREZ, J. M., RUBIO-DÍAZ, S., DHONDT, S., HERNÁNDEZ-ROMERO, D., SÁNCHEZ-SORIANO, J., BEEMSTER, G. T. S., PONCE, M. R. and MICOL, J. L. (2011), Whole organ, venation and epidermal cell morphological variations are correlated in the leaves of Arabidopsis mutants. Plant, Cell & Environment, 34: 2200–2211. doi: 10.1111/j.1365-3040.2011.02415.x
- Issue published online: 9 NOV 2011
- Article first published online: 22 SEP 2011
- Accepted manuscript online: 29 AUG 2011 09:23PM EST
- Received 24 June 2011; accepted for publication 3 August 2011
Figure S1. Effects of seedling density on Arabidopsis growth in vitro. (a) Effects of seedling density on rosette growth of Ler, measured at 21 das (days after stratification) as the area of the best-fitting ellipse. (b) Morphometric analysis of the expansion of the first- and third-node leaves. (c,e) Adaxial epidermis and (d,f) palisade mesophyll of Ler third node vegetative leaves growing at densities of 4 (c,d) and 100 (e,f) plants per Petri dish. Scale bars (c–f): 50 µm.
Figure S2. Rosette morphometry. (a) Dendrogram of rosette samples. The red line indicates Mojena's (M) constant with a value of 2.7, which was used to define the optimal number of clusters for this dataset (see Materials and methods). (b) Scatter plot of the variables representing PC1 (rosette area), PC2 (rosette compactness) and PC3 (rosette evenness). The data are coloured according to clustering. (c) Dendrogram for the first node leaf samples. The red line indicates Mojena's constant with a value of 2.5 (see Materials and methods).
Figure S3. Leaf lamina morphometry. (a) Scatter plots of lamina parameters. The blank diagonal separates the graphs for the first node leaf parameters (right upper area of the rectangle) from those of the third node leaves (left lower area of the rectangle). Full names for variable abbreviations are detailed in Supporting Information Table S2.
Figure S4. Graphic representation of vein parameters. The number of areoles (ALN), number of free-ending veins (FVN) and mean areole area (ALA) per lamina area (LA) (i.e. ALA/LA, FVN/LA and ALA/LA) are represented for the different lines studied, in third-node leaves (L3). Green bars represent the wild-type accessions Ler, Col-0 and En-2. Red bars represent the extreme values, with their genotype symbols indicated in the table below.
Figure S5. Graphic representation of cell parameters for representative genotypes. (a–d) Histograms for MCA values of lines whose data failed to fit the normal distribution: (a) Ler, (b) ang1-1, (c) elo1-1 and (d) den3. (e,f) Histograms for PMA values of representative genotypes that perfectly fit the log-normal (e, den7) and the gamma (f, ang1-1) distributions. (g,h) Histograms for GCA values of representative genotypes that fit the normal distribution (g, icu12) or the log-logistic distribution (h, ucu1-1).
Table S1. Mutants studied in this work.
Table S2. Morphometric parameters studied in this work.
Table S3. Statistical analysis of rosette parameters.
Table S4. Statistical analysis of first-node leaf lamina parameters.
Table S5. Statistical analysis of third-node leaf lamina parameters.
Table S6. Statistical analysis of third-node leaf palisade mesophyll parameters.
Table S7. Statistical analysis of third-node leaf pavement cell parameters.
Table S8. Statistical analysis of third-node leaf adaxial guard cell area.
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