Equal contributors to this work.
SKP1 is involved in abscisic acid signalling to regulate seed germination, stomatal opening and root growth in Arabidopsis thaliana
Article first published online: 8 DEC 2011
© 2011 Blackwell Publishing Ltd
Plant, Cell & Environment
Volume 35, Issue 5, pages 952–965, May 2012
How to Cite
LI, C., LIU, Z., ZHANG, Q., WANG, R., XIAO, L., MA, H., CHONG, K. and XU, Y. (2012), SKP1 is involved in abscisic acid signalling to regulate seed germination, stomatal opening and root growth in Arabidopsis thaliana. Plant, Cell & Environment, 35: 952–965. doi: 10.1111/j.1365-3040.2011.02464.x
- Issue published online: 4 APR 2012
- Article first published online: 8 DEC 2011
- Accepted manuscript online: 10 NOV 2011 07:52AM EST
- Received 22 August 2011; received in revised form 13 October 2011; accepted for publication 20 October 2011
Abscisic acid (ABA) regulates many aspects of plant development, including seed dormancy and germination, root growth and stomatal closure. Plant SKP1 proteins are subunits of the SCF complex E3 ligases, which regulate several phytohormone signalling pathways through protein degradation. However, little is known about SKP1 proteins participating in ABA signalling. Here, we report that the overexpression of Triticum aestivum SKP1-like 1 (TSK1) in Arabidopsis thaliana (Arabidopsis) resulted in delayed seed germination and hypersensitivity to ABA. The opening of stomatal guard cells and the transcription of several ABA-responsive genes were affected in transgenic plants. In contrast, Arabidopsis skp1-like 1 (ask1)/ask1 ASK2/ask2 seedlings exhibited reduced ABA sensitivity. Furthermore, the transcription of ASK1 and ASK2 was down-regulated in abi1-1 and abi5-1 mutants compared with that in wild type. ASK1 or ASK2 overexpression could rescue or partially rescue the ABA insensitivity of abi5-1 mutants, respectively. Our work demonstrates that SKP1 is involved in ABA signalling and that SKP1-like genes may positively regulate ABA signalling by SCF-mediated protein degradation.