Present address: Science Research Center, Oceanography Section, Kochi University, Akebono-cho 2-5-1, Kochi-shi, Kochi, 780-8520, Japan.
Growth-dependent chemical and mechanical properties of cuticular membranes from leaves of Sonneratia alba
Article first published online: 13 FEB 2012
© 2012 Blackwell Publishing Ltd
Plant, Cell & Environment
Volume 35, Issue 7, pages 1201–1210, July 2012
How to Cite
TAKAHASHI, Y., TSUBAKI, S., SAKAMOTO, M., WATANABE, S. and AZUMA, J.-I. (2012), Growth-dependent chemical and mechanical properties of cuticular membranes from leaves of Sonneratia alba. Plant, Cell & Environment, 35: 1201–1210. doi: 10.1111/j.1365-3040.2012.02482.x
- Issue published online: 11 JUN 2012
- Article first published online: 13 FEB 2012
- Accepted manuscript online: 12 JAN 2012 09:10AM EST
- Received 16 July 2011; accepted for publication 1 January 2012
- cuticular membrane;
- growth dependence;
Chemical and mechanical properties of the leaf cuticular membranes (CMs) of a mangrove, Sonneratia alba J. Smith, were analysed at various leaf development stages to evaluate their tolerance to environmental stress. Our analyses demonstrate that the CMs from leaves of S. alba at different growth stages are generally rich in wax (21.5–25.7%) and cutin (52.4–63.4%) which rapidly accumulate at the early stages of leaf growth, while cutan (4.3–10.3%) and polysaccharide (2.3–7.7%) continuously accumulate throughout growth. Immature CMs are physically weak and highly viscoelastic. However, CMs become strengthened and stiffened during leaf expansion and maturation (by factors of about 1.5 and 2.4, respectively) while their flexibility decreases (68–83% decrease). Finally, the CMs lose their strength at the senescent stage (30–43% decreasement). Correlation analysis between chemical composition and mechanical properties revealed that the cutin matrix is mainly responsible for the high viscoelastic properties of CMs, while wax, cutan and polysaccharide contributed to their elasticity. Wax also affected the strength of the CMs, whereas cutan and polysaccharide showed rigidizing effect. Rapid accumulation of wax and cutin in the CMs after bud burst followed by the mechanical supports of cutan and polysaccharide in an isolateral manner contributed to the remarkable environmental tolerance of S. alba.