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Symptoms of bacterial canker of kiwifruit consisting of darkening and shrivelling along the bark were observed in kiwifruit orchards in Tonkabon, Iran. Longidutinal sections showed darkening of cortical and woody tissues. A fluorescent pseudomonad was consistently isolated from canker tissues. According to biochemical, physiological and pathogenicity tests the causal agent was identified as Pseudomonas syringae pv. syringae. This is the first report of the disease in Iran.