Present address: International Fungal Research & Development Centre, Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, PR China.
Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand
Article first published online: 14 JAN 2008
© 2008 The Authors. Journal compilation © 2008 BSPP
Volume 57, Issue 3, pages 562–572, June 2008
How to Cite
Than, P. P., Jeewon, R., Hyde, K. D., Pongsupasamit, S., Mongkolporn, O. and Taylor, P. W. J. (2008), Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathology, 57: 562–572. doi: 10.1111/j.1365-3059.2007.01782.x
- Issue published online: 14 JAN 2008
- Article first published online: 14 JAN 2008
- Accepted 20 August 2007
Fungal isolates from chilli (Capsicum spp.) fruits in Thailand that showed typical anthracnose symptoms were identified as Colletotrichum acutatum, C. capsici and C. gloeosporioides. Phylogenetic analyses from DNA sequence data of ITS rDNA and β-tubulin (tub2) gene regions revealed three major clusters representing these three species. Among the morphological characters examined, colony growth rate and conidium shape in culture were directly correlated with the phylogenetic groupings. Comparison with isolates of C. gloeosporioides from mango and C. acutatum from strawberry showed that host was not important for phylogenetic grouping. Pathogenicity tests validated that all three species isolated from chilli were causal agents for chilli anthracnose when inoculated onto fruits of the susceptible Thai elite cultivar Capsicum annuum cv. Bangchang. Cross-infection potential was shown by C. acutatum isolates originating from strawberry, which produced anthracnose on Bangchang. Interestingly, only C. acutatum isolates from chilli were able to infect and produce anthracnose on PBC 932, a resistant genotype of Capsicum chinense. This result has important implications for Thai chilli breeding programmes in which PBC 932 is being hybridized with Bangchang to incorporate anthracnose resistance into chilli cultivars.