You have full text access to this OnlineOpen article

Dual control of avirulence in Leptosphaeria maculans towards a Brassica napus cultivar with ‘sylvestris-derived’ resistance suggests involvement of two resistance genes

  1. A. P. Van de Wouw1,*,
  2. S. J. Marcroft2,
  3. M. J. Barbetti3,
  4. Li Hua3,
  5. P. A. Salisbury4,
  6. L. Gout5,
  7. T. Rouxel5,
  8. B. J. Howlett1,
  9. M. H. Balesdent5

Article first published online: 16 DEC 2008

DOI: 10.1111/j.1365-3059.2008.01982.x

Issue

Plant Pathology

Plant Pathology

Volume 58, Issue 2, pages 305–313, April 2009

Additional Information

How to Cite

Van de Wouw, A. P., Marcroft, S. J., Barbetti, M. J., Hua, L., Salisbury, P. A., Gout, L., Rouxel, T., Howlett, B. J. and Balesdent, M. H. (2009), Dual control of avirulence in Leptosphaeria maculans towards a Brassica napus cultivar with ‘sylvestris-derived’ resistance suggests involvement of two resistance genes. Plant Pathology, 58: 305–313. doi: 10.1111/j.1365-3059.2008.01982.x

Author Information

  1. 1

    School of Botany, The University of Melbourne, Vic. 3010;

  2. 2

    Marcroft Grains Pathology P/L, Grains Innovation Park, Horsham, Vic. 3400;

  3. 3

    School of Plant Biology, Faculty of Natural and Agricultural Sciences, the University of Western Australia, Crawley, WA 6009;

  4. 4

    Faculty of Land and Food Resources, The University of Melbourne, Vic. 3010 and Department of Primary Industries, Horsham, Vic. 3401, Australia; and

  5. 5

    INRA, UMR1290 BIOGER-CPP, F-78026 Versailles, France

* *E-mail: apvdw2@unimelb.edu.au

Publication History

  1. Issue published online: 11 MAR 2009
  2. Article first published online: 16 DEC 2008
  3. Published online 16 December 2008

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (760K)

References

  • Ansan-Melayah D, Balesdent MH, Rouxel T, 1995. Genetic characterization of AvrLm1, the first avirulence gene of Leptosphaeria maculans. Phytopathology 85, 1525–9.
  • Attard A, Gout L, Gourgues M et al ., 2002. Analysis of molecular markers genetically linked to the Leptosphaeria maculans avirulence gene AvrLm1 in field populations indicates a highly conserved event leading to virulence on Rlm1 genotypes. Molecular Plant-Microbe Interactions 15, 672–82.
  • Aubertot JN, West JS, Bousset-Vaslin L, Salam MU, Barbetti MJ, Diggle AJ, 2006. Improved resistance management for durable disease control: a case study of phoma stem canker of oilseed rape (Brassica napus). European Journal of Plant Pathology 114, 91–106.
  • Balesdent MH, Attard A, Ansan-Melayah D, Delourme R, Renard M, Rouxel T, 2001. Genetic control and host range of avirulence towards Brassica napus cvs. Quinta and Jet Neuf in Leptosphaeria maculans. Phytopathology 91, 70–6.
  • Balesdent MH, Attard A, Kuhn ML, Rouxel T, 2002. New avirulence genes in the phytopathogenic fungus Leptosphaeria maculans. Phytopathology 92, 1122–33.
  • Balesdent MH, Barbetti MJ, Li H, Sivasithamparam K, Gout L, Rouxel T, 2005. Analysis of Leptosphaeria maculans race structure in a worldwide collection of isolates. Phytopathology 95, 1061–71.
  • Balesdent MH, Louvard K, Pinochet X, Rouxel T, 2006. A large-scale survey of Leptosphaeria maculans occurring on oilseed rape in France. European Journal of Plant Pathology 114, 53–65.
  • Blaise F, Remy E, Meyer M et al ., 2007. A critical assessment of Agrobacterium tumefaciens-mediated transformation as a tool for pathogenicity gene discovery in the phytopathogenic fungus Leptosphaeria maculans. Fungal Genetics and Biology 44, 123–38.
  • Cozijnsen AJ, Howlett BJ, 2003. Characterisation of the mating-type locus of the plant pathogenic ascomycete Leptosphaeria maculans. Current Genetics 43, 351–7.
  • Cozijnsen AJ, Popa KM, Purwantara A, Rolls BD, Howlett BJ, 2000. Genome analysis of the plant pathogenic ascomycete Leptosphaeria maculans; mapping mating type and host specificity loci. Molecular Plant Pathology 1, 293–302.
    Direct Link:
  • Crouch JH, Lewis BG, Mithen RF, 1994. The effect of A genome substitution on the resistance of Brassica napus to infection by Leptosphaeria maculans. Plant Breeding 112, 265–78.
    Direct Link:
  • Delourme R, Chevre AM, Brun H et al ., 2006. Major gene and polygenic resistance to Leptosphaeria maculans in oilseed rape (Brassica napus). European Journal of Plant Pathology 114, 41–52.
  • Fudal I, Ross S, Gout L et al ., 2007. Heterochromatin-like regions as ecological niches for avirulence genes in the Leptosphaeria maculans genome: map-based cloning of AvrLm6. Molecular Plant-Microbe Interactions 20, 459–70.
  • Gout L, Fudal I, Kuhn ML et al ., 2006. Lost in the middle of nowhere: the AvrLm1 avirulence gene of the Dothideomycete Leptosphaeria maculans. Molecular Microbiology 60, 67–80.
    Direct Link:
  • Gout L, Kuhn ML, Vincenot L et al ., 2007. Genome structure impacts molecular evolution at the AvrLm1 avirulent locus of the plant pathogen Leptosphaeria maculans. Environmental Microbiology 9, 2978–92.
    Direct Link:
  • Leflon M, Brun H, Eber F et al ., 2007. Detection, introgression and localization of genes conferring specific resistance to Leptosphaeria maculans from Brassica rapa into B. napus. Theoretical and Applied Genetics 11, 5897–906.
  • Li CX, Cowling WA, 2003. Identification of a single dominant allele for resistance to blackleg in Brassica napus ‘Surpass 400’. Plant Breeding 122, 485–8.
    Direct Link:
  • Li H, Sivasithamparam K, Barbetti MJ, 2003. Breakdown of a Brassica rapa subsp. sylvestris single dominant blackleg resistance gene in B. napus rapeseed by Leptosphaeria maculans field isolates in Australia. Plant Disease 87, 752.
  • Manly KF, 1993. A Macintosh program for storage and analysis of experimental genetic-mapping data. Mammalian Genome 4, 303–13.
  • Purwantara A, Burton WA, Salisbury PA, Howlett BJ, 1998. Reaction of Brassica juncea (Indian mustard) lines to Australian isolates of Leptosphaeria maculans under glasshouse and field conditions. European Journal of Plant Pathology 104, 895–902.
  • Rimmer SR, 2006. Resistance genes to Leptosphaeria maculans in Brassica napus. Canadian Journal of Plant Pathology 28, S288–S297.
  • Rouxel T, Balesdent MH, 2005. The stem canker (blackleg) fungus, Leptosphaeria maculans, enters the genomic era. Molecular Plant Pathology 6, 225–41.
    Direct Link:
  • Rouxel T, Kollmann A, Boulidard L, Mithen R, 1991. Abiotic elicitation of indole phytoalexins and resistance to Leptosphaeria maculans within Brassiceae. Planta 184, 271–8.
  • Rouxel T, Willner E, Coudard L, Balesdent MH, 2003a. Screening and identification of resistance to Leptosphaeria maculans in Brassica napus accessions. Euphytica 133, 219–31.
  • Rouxel T, Penaud A, Pinochet X et al ., 2003b. A ten-year survey of populations of Leptosphaeria maculans in France indicates a rapid adaptation towards the Rlm1 resistance gene in oilseed rape. European Journal of Plant Pathology 109, 871–81.
  • Sexton AC, Howlett BJ, 2000. Characterisation of a cyanide hydratase gene in the phytopathogenic fungus Leptosphaeria maculans. Molecular and General Genetics 263, 463–70.
  • Sprague SJ, Marcroft SJ, Hayden HL, Howlett BJ, 2006a. Major gene resistance to blackleg in Brassica napus overcome within three years of commercial production in Southeastern Australia. Plant Disease 90, 190–8.
  • Sprague SJ, Balesdent MH, Brun H et al ., 2006b. Major gene resistance in Brassica napus (oilseed rape) is overcome by changes in virulence of populations of Leptosphaeria maculans in France and Australia. European Journal of Plant Pathology 114, 33–40.
  • Yu F, Lydiate DJ, Rimmer SR, 2005. Identification of two novel genes for blackleg resistance in Brassica napus. Theoretical and Applied Genetics 110, 969–79.
  • Yu F, Lydiate DJ, Rimmer SR, 2008. Identification and mapping of a third blackleg resistance locus in Brassica napus derived from B. rapa subsp. sylvestris. Genome 51, 64–72.
View Full Article (HTML) Get PDF (760K)