Present address: Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, S7N 0X2, Saskatoon, Saskatchewan, Canada.
UV protectants for Candida oleophila (strain O), a biocontrol agent of postharvest fruit diseases
Article first published online: 8 OCT 2010
© 2010 The Authors. Plant Pathology © 2010 BSPP
Volume 60, Issue 2, pages 288–295, April 2011
How to Cite
Lahlali, R., Raffaele, B. and Jijakli, M. H. (2011), UV protectants for Candida oleophila (strain O), a biocontrol agent of postharvest fruit diseases. Plant Pathology, 60: 288–295. doi: 10.1111/j.1365-3059.2010.02368.x
- Issue published online: 1 MAR 2011
- Article first published online: 8 OCT 2010
- Published online 8 October 2010
- Candida oleophila (strain O);
- Penicillium expansum;
- UV-B protectants
This study investigated the influence of UV-B radiation (280–320 nm) on survival of Candida oleophila strain O, an antagonist yeast that prevents postharvest diseases caused by Botrytis cinerea and Penicillium expansum on apple and pear fruits. Lethal doses (LD50 and LD90) were, respectively, 0·89 and 1·45 Kj m−2 for in vitro exposure and 3·06 and 5·5 Kj m−2 for in vivo exposure. A screening test of UV-B protectants for strain O was also evaluated under in vitro and in vivo conditions. The in vitro results showed that sodium ascorbate (0·1% and 0·01%), riboflavin (0·1%) and uric acid (0·1% and 0·01%) were the most effective and most suitable protectants. However, only riboflavin (0·1%) and uric acid (0·1%) were effective under in vivo conditions. The efficacy obtained with strain O against P. expansum, when subjected to UV-B radiation, was 75·0% and 49·2% for pathogen concentrations of 105 and 106 spores mL−1, respectively. Adding riboflavin to strain O gave a similar efficacy (64·2%). Applying strain O together with uric acid (0·1%) was less active (47·7%). Nonetheless, its efficacy when applied with the antioxidants sodium ascorbate (71·1%) or ascorbic acid (82·5%) was the greatest. Riboflavin and uric acid were the most cost-effective protectants, and could be included in the final formulation of strain O when applied preharvest.