Dita MA, Waalwijk C, Buddenhagen IW, Souza Jr MT, Kema, GHJ, 2010. A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen. Plant Pathology59, 348–357.
On page 353, Dita et al. reported diagnostic primers FocTR4-F(orward) (5′-CACGTTTAAGGTGCCATGAGAG-3′) and FocTR4-R(everse) (5′-CGCACGCCAGGACTGCCTCGTGA-3′) for Fusarium oxysporum f.sp. cubense Tropical Race 4 (TR4) detection. However, the authors used the FocTR4-F primer in combination with two different reverse primers. The shorter version of FocTR4-R (5′-GCCAGGACTGCCTCGTGA-3′) worked with the conditions mentioned in the publication (60°C), but the aforementioned longer version is only selective at higher temperatures (68°C).