Genetic population structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil

Authors

  • P. Astolfi,

    1. Departamento de Fitossanidade, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 7712, 9154000 Porto Alegre, RS, Brazil
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  • M. M. Reynoso,

    1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36, Km 601 (5800), Río Cuarto, Córdoba, Argentina
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  • M. L. Ramirez,

    1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36, Km 601 (5800), Río Cuarto, Córdoba, Argentina
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  • S. N. Chulze,

    1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36, Km 601 (5800), Río Cuarto, Córdoba, Argentina
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  • T. C. A. Alves,

    1. Departamento de Agronomia, Universidade Estadual de Maringá, Av. Colombo, 5790-Bloco I 45, 87020900 PR, Brazil
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  • D. J. Tessmann,

    1. Departamento de Agronomia, Universidade Estadual de Maringá, Av. Colombo, 5790-Bloco I 45, 87020900 PR, Brazil
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  • E. M. Del Ponte

    Corresponding author
    1. Departamento de Fitossanidade, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 7712, 9154000 Porto Alegre, RS, Brazil
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E-mail: emerson.delponte@ufrgs.br

Abstract

A sample of 140 Fusarium graminearum isolates from Rio Grande do Sul, Brazil, representing three populations at least 150 km from one another, were examined for trichothecene genotype based on PCR amplification of portions of the Tri3 and Tri12 genes and a species-specific (Fg16F/R) primer pair. Genetic diversity was assessed in a sample of 103 F. graminearum lineage 7 (F. graminearum sensu stricto) isolates using amplified fragment length polymorphism (AFLP) markers. The 15-ADON genotype was dominant, followed by the NIV genotype (2–18% prevalence), across all three populations. All NIV-type isolates were in lineage 2 (F. meridionale) and all 15-ADON-type isolates were in lineage 7. Isolates with the same haplotype were rare and genotypic diversity was uniformly high (≥98% of the count), suggesting that recombination has played a significant role. The number of migrants (Nm) was estimated between 5 and 6 across all loci and all populations, but the high frequency of private alleles (up to 30%) suggests a historical, rather than contemporary, gene flow. Regarding linkage disequilibrium, 0·8, 1·5 and 2·2% of the locus pairs from the three populations were in disequilibrium, which is lower than values reported in other locations. Thus, Brazilian populations differ from those found in Europe, North America and most of Asia in the presence of a significant frequency (7·8%) of isolates of the NIV genotype in lineage 2.

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