A Phytophthora conserved transposon-like DNA element as a potential target for soyabean root rot disease diagnosis

Figure S1 The position of the A3aProelement in the Avr3a gene.

Figure S2 1% agarose gel electrophoresis ofpolymerase chain reaction (PCR) products using Phytophthorasojae specific primers TrapF1/TrapR1 (at least 6 replicates).(a) PCR amplification products from P.  sojaestrain and isolates of Phytophthora spp. (b) PCRamplification products from P.  sojae strain andisolates of true fungi. (c) PCR amplification products fromdifferent P.  sojae strains.

Figure S3 Sensitivity of standard PCR withPhytophthora sojae DNA from zoospores (upper row) and oospores (lower row). PCR with TrapF1/TrapR1 primers detected the pathogen at the level of a single oospore.

Figure S4 Correlation coefficients assessed forPhytophthora sojae using real-time PCR amplification with primers TrapF1 and TrapR1 (at least 6 replicates).

Figure S5 Nested PCR with TrapF1/TrapR1 and TrapF2/TrapR2 primers amplification of DNA extracted from infested field soils (at least 6 replicates).

Figure S6 Polymerase chain reaction (PCR)products using previously published primers PS1/PS2 ofPhytophthora sojae (at least 6 replicates). Annealingtemperature: (a) 60°C; (b) 66°C.

Filename	Format	Size	Description
ppa2672_sm_FigS1.pdf		144K	Supporting info item
ppa2672_sm_FigS2.pdf		121K	Supporting info item
ppa2672_sm_FigS3.pdf		431K	Supporting info item
ppa2672_sm_FigS4.pdf		55K	Supporting info item
ppa2672_sm_FigS5.pdf		70K	Supporting info item
ppa2672_sm_FigS6.pdf		153K	Supporting info item

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