Demonstration of Enolase Activity Connected to the Brain-Specific Protein 14-3-2

Authors

  • ELISABETH BOCK M.D,

    Corresponding author
    1. Psychochemistry Institute, Rigshospitalet, and Institute of Forensic Medicine, Serological Department, University of Copenhagen, Denmark
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  • J. DISSING

    1. Psychochemistry Institute, Rigshospitalet, and Institute of Forensic Medicine, Serological Department, University of Copenhagen, Denmark
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Protein Laboratory, Sigurdsgade 34, DK-2200 Copenhagen, Denmark

Abstract

An enzyme activity, enolase (2-phospho-D-glycerate hydro-lyase, E.C. 4.2.1.11), was shown to be connected to the brain-specific protein 14.3.2. Specific staining methods were used on immuno precipitates of the protein. The enzyme activity was strongly inhibited by the addition of fluoride in the presence of phosphate. Metal ion binding capacity of the protein was shown by means of an auto radiographic method.

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