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Enzyme Characterization in Quantitative Immunoelectrophoresis
An Enzymological Study of Human Serum Esterases
Article first published online: 9 FEB 2009
DOI: 10.1111/j.1365-3083.1975.tb03807.x
© 1975 Blackwell Publishing Ltd
1365-3083/asset/cover.gif?v=1&s=851263375540b207304bed4ad505b46ca5c4242b "Scandinavian Journal of Immunology")
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How to Cite
BROGREN, C.-H. and BøG-HANSEN, T. C. (1975), Enzyme Characterization in Quantitative Immunoelectrophoresis. Scandinavian Journal of Immunology, 4: 37–51. doi: 10.1111/j.1365-3083.1975.tb03807.x
Publication History
- Issue published online: 9 FEB 2009
- Article first published online: 9 FEB 2009
- Received 5 December 1974
- Abstract
- References
- Cited By
Carboxylic ester hydrolases in human serum were identified, characterized, and quantified by means of crossed and rocket immunoelectrophoresis. Two types of esterase were found, arylesterase (EC 3.1.1.2) and cholinesterase (EC 3.1.1.8), exhibiting differences in specificity for several histochemical substrates. α-naphthyl acetate and β-naphthyl acetate were used to distinguish between the two enzymes. Arylesterase seemed to interact specifically with aJipoprotein. Quantitation of enzyme protein and correlation with enzyme activity is described. The experiments illustrate general methodological problems concerning immunochemical characterization of enzymes.