Thin-Layer Gel Filtration Immunoelectrophoresis


The Protein Laboratory, Sigurdsgade 34, DK-2200 Copenhagen, Denmark


A method that directly combines separation of proteins by thin-layer gel filtration (TLG) with quantitative immunoelectrophoresis is described. All the operations are performed in monolayer gels on a single glass plate. The resolving power of the method is comparable to that of an ordinary column technique monitored by fused rocket immunoelectrophoresis.