The Protective Effect of β1–3D-Glucan-Derivatized Plastic Beads against Escherichia coli Infection in Mice

Authors

  • R. SELJELID,

    Corresponding author
    1. Instilute of Medical Biology, University of Tormsø. Tromsø, Norway, and Department of Chemistry and Molecular Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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  • L.-T. RASMUSSEN,

    1. Instilute of Medical Biology, University of Tormsø. Tromsø, Norway, and Department of Chemistry and Molecular Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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  • O. LARM,

    1. Instilute of Medical Biology, University of Tormsø. Tromsø, Norway, and Department of Chemistry and Molecular Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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  • J. HOFFMAN

    1. Instilute of Medical Biology, University of Tormsø. Tromsø, Norway, and Department of Chemistry and Molecular Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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R. Seljelid, IMB, Box 977, 9001 Tromso, Norway

Abstract

Pretreatment with β-1, 3-D-glucan-derivated plastic beads conferred strong protection against Escherichia coli infection in mice. The protective effect showed a dose-response relationship to the amount of beads injected and was dependent on the time point of the injection relative to the infection with E. coli. A similar protection could be obtained in nude mice. Experiments with radioactively labelled bacteria as well as beads indicated a systemic effect of the beads. macrophages extracted from animals treated with glucan plastic beads appeared highly stimulated. This was also true of cells that did not contain beads and presumably therefore not glucan, which seems to indicate a soluble stimulatory factor.

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