Expression and Secretion of T-Cell Receptor Vα and Vβ Domains using Escherichia coli as a Host

Authors

  • E. S. WARD

    Corresponding author
    1. Cancer Immunobiology Center and Department of Microbiology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
      E. Sally Ward, Cancer Immunobiology Center and Department of Microbiology. The University of Texas Southwestern Medical Center at Dallas. 5323 Harry Hines Bird. Dallas. TX 75235-8576. USA
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E. Sally Ward, Cancer Immunobiology Center and Department of Microbiology. The University of Texas Southwestern Medical Center at Dallas. 5323 Harry Hines Bird. Dallas. TX 75235-8576. USA

Abstract

An expression system for the production of recombinant T-cell receptor (TCR) variable domains would, inter alia, allow structural studies lo be carried out and provide protein for the generation of anti-clonotypic antibodies, in this report the Vα and Vβ domain genes have been isolated from a T-cell hybridoma which is associated with the pathogenesis of experimental allergic encephalomyelitis (EAE) In the H-2 mouse. These have been expressed as secreted domains in Escherichia coli, using secretion vectors previously used for the production of immunoglobulin fragments. Both Vα and Vβ domains are secreted in milligram quantities into the culture supernatant, although the levels of the Vα domain are about 10-20 Ibid higher than those of the Vβ domain. This expression system offers a rapid route for the production of recombinant TCRs in soluble form.

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