BASIC IMMUNOLOGY

Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma

  1. D. R. Martins1,
  2. A. C. Pelizon1,
  3. S. F. G. Zorzella-Pezavento1,
  4. J. Seger1,
  5. R. R. Santos Junior2,
  6. D. M. Fonseca2,
  7. L. A. Justulin Jr3,
  8. C. L. Silva2,
  9. A. Sartori1

Article first published online: 1 MAR 2011

DOI: 10.1111/j.1365-3083.2011.02510.x

Issue

Scandinavian Journal of Immunology

Scandinavian Journal of Immunology

Volume 73, Issue 4, pages 293–300, April 2011

Additional Information

How to Cite

Martins, D. R., Pelizon, A. C., Zorzella-Pezavento, S. F. G., Seger, J., Santos Junior, R. R., Fonseca, D. M., Justulin Jr, L. A., Silva, C. L. and Sartori, A. (2011), Exposure to Mycobacterium avium Decreases the Protective Effect of the DNA Vaccine pVAXhsp65 Against Mycobacterium tuberculosis-Induced Inflammation of the Pulmonary Parenchyma. Scandinavian Journal of Immunology, 73: 293–300. doi: 10.1111/j.1365-3083.2011.02510.x

Author Information

  1. 1

    Department of Microbiology and Immunology, Biosciences Institute, Univ Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil

  2. 2

    Department of Biochemistry and Immunology, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil

  3. 3

    Institute of Biologic and Natural Science, Federal University of Triângulo Mineiro (UFMT), Uberaba, Minas Gerais, Brazil

*A. Sartori, Department of Microbiology and Immunology, Biosciences Institute, Univ Estadual Paulista (UNESP), Distrito de Rubião Júnior, s/n, Botucatu, São Paulo 18618-000, Brazil. E-mail: sartori@ibb.unesp.br

Publication History

  1. Issue published online: 1 MAR 2011
  2. Article first published online: 1 MAR 2011
  3. Accepted manuscript online: 7 JAN 2011 10:16AM EST
  4. Received 24 August 2010; Accepted in revised form 30 December 2010

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Abstract

This work investigated the effect of previous Mycobacterium avium exposure on the protective ability of the DNA vaccine pVAXhsp65 against inflammation in the pulmonary parenchyma. BALB/c mice were presensitized with heat-killed M. avium and then immunized with three doses of pVAXhsp65 prior to challenge with Mycobacterium tuberculosis. M. avium sensitization induced high levels of spontaneous IL-5 production that were concomitant with a positive delayed-type hypersensitivity reaction; antigen-specific IFN-γ production was also observed upon splenocyte stimulation. Prior exposure to M. avium resulted in altered cytokine and antibody production induced by immunization with pVAXhsp65; instead of a Th1 response, vaccinated mice previously exposed to M. avium developed a strong Th2 response. This switch to a Th2 response coincided with the loss of the anti-inflammatory effect of pVAXhsp65 vaccination previously observed in the pulmonary parenchyma of mice infected with M. tuberculosis. These results suggest that exposure to environmental mycobacteria can modulate immune responses induced by mycobacterial vaccines other than bacillus Calmette–Guérin.

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