The Ediacaran to lowermost Cambrian successions of south-eastern Uruguay preserve an unusual and significant record of deposits generated during the Gondwana assembly (ca 590 to 535 Ma). This study presents a review of data obtained through extensive field-based mapping coupled with detailed sedimentology and stratigraphy of key formations. The geological units within the study area consist of the Maldonado Group (Playa Hermosa, Las Ventanas and San Carlos formations), the Arroyo del Soldado Group (Yerbal, Polanco Limestones, Barriga Negra and Cerro Espuelitas formations) and the Arroyo de la Pedrera Group (Piedras de Afilar and Cerro Victoria formations). The Maldonado Group is characterized by a glacially influenced volcanogenic-sedimentary sequence with ice-rafted debris and dropstones in the Playa Hermosa and Las Ventanas formations. The Arroyo del Soldado Group is a mixed siliciclastic-carbonate succession, mainly represented by an intercalation of basal pink dolostones, banded siltstones, rhythmites of dolostone-limestone, iron formations, cherts and conglomerates. Carbonates in the Polanco Limestones Formation are characterized by a negative δ13C excursion up to −3·26‰ PeeDeeBelemnite. The Arroyo de la Pedrera Group consists of quartz arenites and stromatolitic/oolitic dolostones. Preliminary data indicate that the Precambrian–Cambrian could be contained within or at the base of this group. The entire succession is almost 6000 m thick, and contains a rich fossil assemblage composed of organic-walled microfossils and small shelly fauna, including the index fossil Cloudina riemkeae. The stratigraphic and chemostratigraphic features are suggestive of a Gaskier age (ca 580 Ma) for the basal glacial-related units. In this scenario, the results show the importance of lithostratigraphic, biostratigraphic and chemostratigraphic data of these Ediacaran units in the global correlation of terminal Proterozoic sedimentary rocks.