Present address: Department of Biology, Pennsylvania State University, University Park, PA 16802, USA.
Differential mRNA translation contributes to gene regulation under non-stress and dehydration stress conditions in Arabidopsis thaliana
Article first published online: 13 APR 2004
The Plant Journal
Volume 38, Issue 5, pages 823–839, June 2004
How to Cite
Kawaguchi, R., Girke, T., Bray, E. A. and Bailey-Serres, J. (2004), Differential mRNA translation contributes to gene regulation under non-stress and dehydration stress conditions in Arabidopsis thaliana. The Plant Journal, 38: 823–839. doi: 10.1111/j.1365-313X.2004.02090.x
- Issue published online: 13 APR 2004
- Article first published online: 13 APR 2004
- Received 8 January 2004; revised 11 February 2004; accepted 23 February 2004.
- ribosome loading;
- water-deficit stress;
- dehydration stress;
- translational control
Translational regulation was evaluated for over 2000 genes by measurement of the proportion of individual mRNA species in polysomal (PS) complexes in leaves of non-stressed and moderately dehydration-stressed Arabidopsis. The amount of each mRNA in polysomes ranged from 23 to 97% in non-stressed leaves and was significantly reduced for a large portion of the genes (71%) in response to dehydration. The effect of dehydration on translational status varied extensively between mRNA species. Sixty per cent of the dehydration-inducible mRNAs with twofold or greater increase in abundance maintained PS levels in response to water-deficit stress, while 40% showed impaired ribosome loading (RL). PS association declined significantly for 92% of the mRNAs that displayed a strong decrease in abundance, indicating a relationship between translation and decreased gene transcription and/or mRNA stability. Interestingly, many mRNAs that encode proteins of similar biological function displayed coordinate translational regulation. Thus, the abundance of PS mRNA may provide a more accurate estimate of gene expression than total cellular mRNA because of extensive differential translational regulation.