Present address: Laboratoire Arago, UMR7628 CNRS, Université Pierre et Marie Curie, BP 44, F-66651 Banyuls sur Mer cedex, France.
Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins
Article first published online: 22 SEP 2004
The Plant Journal
Volume 40, Issue 3, pages 386–398, November 2004
How to Cite
Damme, D. V., Bouget, F.-Y., Poucke, K. V., Inzé, D. and Geelen, D. (2004), Molecular dissection of plant cytokinesis and phragmoplast structure: a survey of GFP-tagged proteins. The Plant Journal, 40: 386–398. doi: 10.1111/j.1365-313X.2004.02222.x
- Issue published online: 22 SEP 2004
- Article first published online: 22 SEP 2004
- Received 14 July 2003; accepted 3 August 2004.
- cell division;
To identify molecular players implicated in cytokinesis and division plane determination, the Arabidopsis thaliana genome was explored for potential cytokinesis genes. More than 100 open reading frames were selected based on similarity to yeast and animal cytokinesis genes, cytoskeleton and polarity genes, and Nicotiana tabacum genes showing cell cycle-controlled expression. The subcellular localization of these proteins was determined by means of GFP tagging in tobacco Bright Yellow-2 cells and Arabidopsis plants. Detailed confocal microscopy identified 15 proteins targeted to distinct regions of the phragmoplast and the cell plate. EB1- and MAP65-like proteins were associated with the plus-end, the minus-end, or along the entire length of microtubules. The actin-binding protein myosin, the kinase Aurora, and a novel cell cycle protein designated T22, accumulated preferentially at the midline. EB1 and Aurora, in addition to other regulatory proteins (homologs of Mob1, Sid1, and Sid2), were targeted to the nucleus, suggesting that this organelle operates as a coordinating hub for cytokinesis.