Present address: Faculty of Plant Biotechnology, Dong-A University, Hadan 2-dong 840, Saha-gu, Busan 604-714, Korea.
The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Article first published online: 2 JUN 2005
The Plant Journal
Volume 43, Issue 1, pages 68–78, July 2005
How to Cite
Horiguchi, G., Kim, G.-T. and Tsukaya, H. (2005), The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana. The Plant Journal, 43: 68–78. doi: 10.1111/j.1365-313X.2005.02429.x
- Issue published online: 2 JUN 2005
- Article first published online: 2 JUN 2005
- Received 6 February 2005; revised 2 April 2005; accepted 14 April 2005.
- Arabidopsis thaliana;
- cell proliferation;
- leaf width;
The development of the flat morphology of leaf blades is dependent on the control of cell proliferation as well as cell expansion. Each process has a polarity with respect to the longitudinal and transverse axes of the leaf blade. However, only a few regulatory components of these processes have been identified to date. We have characterized two genes from Arabidopsis thaliana: ANGUSTIFOLIA3 (AN3), which encodes a homolog of the human transcription coactivator SYT, and GROWTH-REGULATING FACTOR5 (AtGRF5), which encodes a putative transcription factor. AN3 is identical to GRF-INTERACTING FACTOR1 (AtGIF1). The an3 and atgrf5 mutants exhibit narrow-leaf phenotypes due to decreases in cell number. Conversely, cell proliferation in leaf primordia is enhanced and leaves grow larger than normal when AN3 or AtGRF5 is overexpressed. Both genes are expressed in leaf primordia, and in the yeast two-hybrid assay, the gene products were found to interact with each other through their N-terminal domains. These results suggest that AN3 and AtGRF5 act together and are required for the development of appropriate leaf size and shape through the promotion and/or maintenance of cell proliferation activity in leaf primordia.