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Figure S1. Overview of gene expression in torpedo, cotyledon and tps1 embryos using Mapman mapping of metabolic genes (Thimm et al., 2004). Pathways marked in red illustrate similar trends between bent cotyledon and tps1 compared to torpedo.

(a) Functional categories assigned in each location of the map. The colour code used in (b), (c), and (d) saturates at eightfold changes and red indicates decreased expression while blue indicates overexpression. Genes absent in both sets are marked in grey. (b) Comparison of gene expression between wild type bent cotyledon and torpedo embryos. (c) Comparison between tps1 and torpedo embryos. (d) Comparison between tps1 and bent cotyledon embryos.

Figure S2. Expression of phosphoenolpyruvate carboxykinase and isocytrate lyase genes in wild type and tps1 embryos. β-glucuronidase activity controlled by the promoter of PEPCK (a) or ICL (b) in wild type embryos at 18 DAF (left), tps1 embryos 18 DAF (centre), and wild type torpedo embryos 7 DAF (right).

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