The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short- and long-term exposure to low temperature

Authors

  • Estelle Goulas,

    1. Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-901 87 Umeå, Sweden,
    2. UMR INRA-USTL SADV ‘Stress Abiotiques et Différenciation des Végétaux cultivés’, Bat SN2, 3eétage, Université des Sciences et Technologies de Lille 1, F-59655 Villeneuve d'Ascq cedex, France,
    Search for more papers by this author
    • The first two authors contributed equally to this paper, and this work is the result of an equal collaboration between the laboratories of the last two authors.

  • Maria Schubert,

    1. Department of Medical Nutrition, Karolinska Institute, S-141 86 Huddinge, Sweden,
    2. School of Life Sciences, Södertörns University College, S-141 89 Huddinge, Sweden, and
    Search for more papers by this author
    • The first two authors contributed equally to this paper, and this work is the result of an equal collaboration between the laboratories of the last two authors.

  • Thomas Kieselbach,

    1. Department of Biochemistry, Institute of Chemistry, Umeå University, S-901 87 Umeå, Sweden
    Search for more papers by this author
  • Leszek A. Kleczkowski,

    1. Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-901 87 Umeå, Sweden,
    Search for more papers by this author
  • Per Gardeström,

    1. Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-901 87 Umeå, Sweden,
    Search for more papers by this author
  • Wolfgang Schröder,

    1. Department of Biochemistry, Institute of Chemistry, Umeå University, S-901 87 Umeå, Sweden
    Search for more papers by this author
    • The first two authors contributed equally to this paper, and this work is the result of an equal collaboration between the laboratories of the last two authors.

  • Vaughan Hurry

    Corresponding author
    1. Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-901 87 Umeå, Sweden,
    Search for more papers by this author
    • The first two authors contributed equally to this paper, and this work is the result of an equal collaboration between the laboratories of the last two authors.


Summary

Cold acclimation and over-wintering by herbaceous plants are energetically expensive and are dependent on functional plastid metabolism. To understand how the stroma and the lumen proteomes adapt to low temperatures, we have taken a proteomic approach (difference gel electrophoresis) to identify proteins that changed in abundance in Arabidopsis chloroplasts during cold shock (1 day), and short- (10 days) and long-term (40 days) acclimation to 5°C. We show that cold shock (1 day) results in minimal change in the plastid proteomes, while short-term (10 days) acclimation results in major changes in the stromal but few changes in the lumen proteome. Long-term acclimation (40 days) results in modulation of the proteomes of both compartments, with new proteins appearing in the lumen and further modulations in protein abundance occurring in the stroma. We identify 43 differentially displayed proteins that participate in photosynthesis, other plastid metabolic functions, hormone biosynthesis and stress sensing and signal transduction. These findings not only provide new insights into the cold response and acclimation of Arabidopsis, but also demonstrate the importance of studying changes in protein abundance within the relevant cellular compartment.

Ancillary