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EDM2 is required for RPP7-dependent disease resistance in Arabidopsis and affects RPP7 transcript levels

  1. Thomas Eulgem1,2,*,
  2. Tokuji Tsuchiya2,
  3. Xiao-Jun Wang3,
  4. Britt Beasley1,
  5. Alayne Cuzick4,†,
  6. Mahmut Tör4,
  7. Tong Zhu5,
  8. John M. McDowell3,
  9. Eric Holub4,
  10. Jeffery L. Dangl1,6

Article first published online: 24 JAN 2007

DOI: 10.1111/j.1365-313X.2006.02999.x

Issue

The Plant Journal

The Plant Journal

Volume 49, Issue 5, pages 829–839, March 2007

Additional Information

How to Cite

Eulgem, T., Tsuchiya, T., Wang, X.-J., Beasley, B., Cuzick, A., Tör, M., Zhu, T., McDowell, J. M., Holub, E. and Dangl, J. L. (2007), EDM2 is required for RPP7-dependent disease resistance in Arabidopsis and affects RPP7 transcript levels. The Plant Journal, 49: 829–839. doi: 10.1111/j.1365-313X.2006.02999.x

Author Information

  1. 1

    Department of Biology, CB#3280 University of North Carolina at Chapel Hill, NC 27599, USA,

  2. 2

    Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California at Riverside, CA 92521, USA,

  3. 3

    Department of Plant Pathology, Physiology, and Weed Science, and Fralin Biotechnology Center, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0346, USA,

  4. 4

    Warwick-HRI, University of Warwick, Warwick CV35 9EF, UK,

  5. 5

    Syngenta Biotechnology, Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709, USA, and

  6. 6

    Department of Microbiology and Immunology, Curriculum in Genetics, Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, NC 27599, USA

  1. †

    Present address: Alayne Cuzick, Plant-Pathogen Interactions Division, Rothamsted Research, Harpenden, Herts. AL5 2JQ, UK.

* (fax +1 951 827 4437; e-mail thomas.eulgem@ucr.edu).

  • †

    Present address: Alayne Cuzick, Plant-Pathogen Interactions Division, Rothamsted Research, Harpenden, Herts. AL5 2JQ, UK.

Publication History

  1. Issue published online: 2 FEB 2007
  2. Article first published online: 24 JAN 2007
  3. Received 4 July 2006; revised 15 October 2006; accepted 18 October 2006.

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Keywords:

  • Arabidopsis thaliana;
  • Hyaloperonospora parasitica;
  • disease resistance gene;
  • EDM2 (enhanced downy mildew 2)-like proteins (ELPs);
  • plant homeodomain (PHD) finger

Summary

Specific disease resistance of Arabidopsis thaliana against the Hyaloperonospora parasitica isolate Hiks1 (HpHiks1) is mediated by RPP7. Although this disease resistance gene encodes a typical nucleotide binding site leucine-rich repeat (NB-LRR) disease resistance protein, its function is independent of the defense hormone salicylic acid and most known genes required for plant immune responses. We identified EDM2 (enhanced downy mildew 2) in a genetic screen for RPP7 suppressors. Mutations of EDM2 phenocopy RPP7 mutations, but do not affect other tested disease resistance genes. We isolated EDM2 by map-based cloning. The predicted EDM2 protein is structurally unrelated to previously identified components of the plant immune system, bears typical features of transcriptional regulators, including plant homeodomain (PHD)-finger-like domains, and defines a plant-specific protein family. In edm2 mutants both constitutive and HpHiks1-induced RPP7 transcript levels are reduced, suggesting that EDM2 is either a direct or an indirect regulator of RPP7 expression. Microarray analyses defined a set of defense-associated genes, the expression of which is suppressed during successful HpHiks1 colonization of either rpp7 or edm2 plants. This transcriptional phenotype is counteracted by an EDM2/RPP7-dependent mechanism.

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