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Figure S1. Transgenic late-heart embryos expressing the Rub-3b silencing trigger from the DR5rev promoter. Length of silenced area in hypocotyl and total embryo length of each embryo were measured using Canvas 9, and normalized to the corresponding scale in each picture. Figure S2. Transgenic early-torpedo embryos expressing the Rub-3b silencing trigger from the DR5rev promoter. Length of silenced area in hypocotyl and total embryo length of each embryo were measured using Canvas 9, and normalized to the corresponding scale in each picture. Figure S3. Transgenic mid-torpedo embryos expressing the Rub-3b silencing trigger from the DR5rev promoter. Length of silenced area in hypocotyl and total embryo length of each embryo were measured using Canvas 9, and normalized to the corresponding scale in each picture. Figure S4. Control experiments. (A) dsRNA GFP induced silencing of early-torpedo GFP142 embryo. (B) Control, untreated GFP142 embryo. (C and D) GFP-miR171.1 late heart and mid torpedo silenced embryos in the sde1 background. (E) Line 2-3 seedling cotyledon exhibiting photobleaching at cotyedon tips (arrow). (F) Small RNA Northern blot detecting 21 nt siRNA for Rub-3b sequence. Lanes 1: control, 2 and 3: line 2-3 with strong phenotype, 4: line 2-5. Lower panel shows loading control. Bar: 50 μm.

FilenameFormatSizeDescription
TPJ_3073_sm_FigS1.pdf1286KSupporting info item
TPJ_3073_sm_FigS2.pdf1404KSupporting info item
TPJ_3073_sm_FigS3.pdf1303KSupporting info item
TPJ_3073_sm_FigS4.pdf1192KSupporting info item

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