Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine

Authors

  • Xing Rong Wu,

    1. Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA, and
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  • Alexander Kenzior,

    1. Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA, and
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  • David Willmot,

    1. Plant Sciences Unit; University of Missouri-Columbia, Columbia, MO 65211, USA
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    • Present address: Agilent Technologies, 2850 Centerville Rd, Wilmington, DE 19808, USA.

  • Sarah Scanlon,

    1. Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA, and
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  • Zhihong Chen,

    1. Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA, and
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    • Present address: Nichols Institute Diagnostics, 1311 Calle Batido, San Clemente, CA 92673, USA.

  • Andrey Topin,

    1. Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA, and
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    • Present address: Sequoia Pharmaceuticals, Inc.,401 Professional Dr, Suite 100 Gaithersburg, MD 20879, USA.

  • Shao Hua He,

    1. Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA, and
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    • Present address: Analytical Services, University of Missouri-Columbia, Columbia, MO, USA.

  • Ashley Acevedo,

    1. Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA, and
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  • William R. Folk

    Corresponding author
    1. Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA, and
      (fax +1 573 884 4808; e-mail folkw@missouri.edu).
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(fax +1 573 884 4808; e-mail folkw@missouri.edu).

Summary

The Arabidopsis thaliana lysyl tRNA synthetase (AtKRS) structurally and functionally resembles the well-characterized prokaryotic class IIb KRS, including the propensity to aminoacylate tRNALys with suboptimal identity elements, as well as non-cognate tRNAs. Transient expression of AtKRS in carrot cells promotes aminoacylation of such tRNAs in vivo and translational recoding of lysine at nonsense codons. Stable expression of AtKRS in Zea mays causes translational recoding of lysine into zeins, significantly enriching the lysine content of grain.

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