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Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine
Article first published online: 8 APR 2007
The Plant Journal
Volume 50, Issue 4, pages 627–636, May 2007
How to Cite
Wu, X. R., Kenzior, A., Willmot, D., Scanlon, S., Chen, Z., Topin, A., He, S. H., Acevedo, A. and Folk, W. R. (2007), Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine. The Plant Journal, 50: 627–636. doi: 10.1111/j.1365-313X.2007.03076.x
- Issue published online: 25 APR 2007
- Article first published online: 8 APR 2007
- Received 1 October 2006; revised 23 December 2006; accepted 16 January 2007.
- aminoacyl tRNA synthetase;
- translational reading;
The Arabidopsis thaliana lysyl tRNA synthetase (AtKRS) structurally and functionally resembles the well-characterized prokaryotic class IIb KRS, including the propensity to aminoacylate tRNALys with suboptimal identity elements, as well as non-cognate tRNAs. Transient expression of AtKRS in carrot cells promotes aminoacylation of such tRNAs in vivo and translational recoding of lysine at nonsense codons. Stable expression of AtKRS in Zea mays causes translational recoding of lysine into zeins, significantly enriching the lysine content of grain.