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Figure S1. Ambient temperature during the experimental period. The red line and the blue line represent the maximum and minimum temperatures, respectively in degrees Celsius. Figure S2. Semi-quantitative RT-PCR analysis of the expression of selected clones during activity-dormancy cycle. (a) bark storage protein (PU03474), (b) beta-fructosidase (PU04013), (c) histone H4 (PU05693), (d) PttGA20oxidase (PU07797), (e) isocitrate lyase (PU03658 (f) bark storage protein (PU03474), (g) histone H4 (PU05693), (h) isocitrate lyase (PU03658), (i) raffinose synthase (PU03091), (j) beta-amylase (PU03585). Values on the Y-axis are relative expression levels. Table S1. Differentially expressed clones during spring reactivation (Phase 1) and autumn growth cessation and dormancy (Phase 2). Table S1 describes the clones whose expression is significantly altered (See Figure 2 for expression profiles) during the period April to June (phase 1) and June to December (phase 2). The table describes the cluster, PU number, poplar EST corresponding to the PU number (see http://www.populusdb.umu.se for details), closest Arabidopsis gene (AGI number), poplar gene model corresponding to the EST, annotation and relative gene expression level. Poplar gene models sequences were utilized to obtain annotation as described earlier (Sterky et al., 2004). Table S2. Analysis of cambial metabolome during the course of activity-dormancy cycle. GC/TOFMS peaks according to PLS-DA identified as important for explaining the differences between cambial samples at different stages of activity-dormancy cycle. aSpA=peak more abundant in April than June; SpJ=peak more abundant in June than April; SuJ=+June/-August; SuA=-June/+Aug; AuA=+Aug/-Sep; AuS=-Aug/+Sep; WiS=+Sep/-Dec; WiD=-Sep/+Dec; bPeaks are named according to UPSC- in-house mass spectra library. cAnnotation of peaks were performed by comparing mass spectrum and retention index (RI) with the dUPSC in-house mass spectra library or the mass spectra library maintained by the Max Planck Institute (MPI) in Golm (http://csbdb.mpimp-golm.mpg.de/csbdb/gmd/gmd.html). ePeaks annotated or classified according “M000000…” are identical or similar to non-annotated mass spectra in the MPI-library. Naming refers to MPI-spectra numbering. UPSC mass spectra will shortly be available for download on UPSC homepage (http://www.upsc.se). Table S3. Analysis of the core cell cycle gene expression during spring reactivation and autumn growth cessation and dormancy. Table S3 describes the poplar cell cycle genes represented on the array. Poplar homologs of Arabidopsis cell cycle genes were identified by comparing the sequences of poplar clones represented on the array with that of Arabidopsis core cell cycle genes described by Menges et al. (2005) using tblastX (Altschul et al., 1990). The cell cycle genes whose expression was significantly altered in phase 1 or Phase 2 is indicated with a cross in the table and their expression profiles are shown in figure 4. Table S4. Analysis of the expression of cold hardiness related genes during the course of activity-dormancy cycle. Table S4 describes the low temperature induced and cold hardiness related genes in poplar. Genes compiled in this table were obtained by: (i) identifying poplar homologs of Arabidopsis genes shown to be low temperature induced by (Vogel et al., 2005), (ii) poplar genes induced by low temperature or by overexpression of AtCBF1 (Benedict et al., 2006) and (iii) poplar genes homologous to those that have been implicated earlier in cold hardiness related processes in tree species (Rowland and Arora, 1997; Renaut et al., 2004). Where known, specific classes of genes are color coded. Expression profiles of clones in bold letters are shown in figure 5 and the cluster to which they belong are indicated in the table. ReferencesAltschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol., 215, 403-410. Sterky, F., Bhalerao, R.R., Unneberg, P., Segerman, B., Nilsson, P., Brunner, A.M., Charbonnel-Campaa, L., Jonsson-Lindvall, J., Tandre, K., Strauss, S.H., Sundberg, B., Gustafsson, P., Uhl�n, M., Bhalerao, R.P., Nilsson, O., Sandberg, G., Karlsson, J., Lundeberg, J. and Jansson, S. (2004) A populus EST resource for plant functional genomics. Proc Natl Acad Sci USA, 101, 13951-13956.

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TPJ_3077_sm_FigS1.pdf156KSupporting info item
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TPJ_3077_sm_Tables.zip516KSupporting info item

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