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Characterization of seed-specific benzoyloxyglucosinolate mutations in Arabidopsis thaliana

  1. Daniel J. Kliebenstein1,†,
  2. John C. D’Auria2,†,
  3. Aditi S. Behere3,‡,
  4. Jae Hak Kim3,
  5. Kevin L. Gunderson4,
  6. John N. Breen5,§,
  7. Grace Lee4,¶,
  8. Jonathan Gershenzon2,
  9. Robert L. Last5,‖,
  10. Georg Jander3

Article first published online: 25 JUL 2007

DOI: 10.1111/j.1365-313X.2007.03205.x

Issue

The Plant Journal

The Plant Journal

Volume 51, Issue 6, pages 1062–1076, September 2007

Additional Information

How to Cite

Kliebenstein, D. J., D’Auria, J. C., Behere, A. S., Kim, J. H., Gunderson, K. L., Breen, J. N., Lee, G., Gershenzon, J., Last, R. L. and Jander, G. (2007), Characterization of seed-specific benzoyloxyglucosinolate mutations in Arabidopsis thaliana. The Plant Journal, 51: 1062–1076. doi: 10.1111/j.1365-313X.2007.03205.x

Author Information

  1. 1

    Department of Plant Sciences, University of California-Davis, One Shields Ave, Davis, CA 95616, USA

  2. 2

    Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans-Knoll Strasse 8, D-07745 Jena, Germany

  3. 3

    Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA

  4. 4

    Illumina, Inc. 9885 Towne Centre Dr., San Diego, CA 92121, USA

  5. 5

    Cereon Genomics, 45 Sidney Street, Cambridge MA 02139, USA

  1. †

    DJK and JCD contributed equally to this work and should be considered co-first authors.

  2. Present address: Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.

  3. ‡

    Present address: Codon Devices, One Kendall Square, Cambridge, MA 02139, USA.

  4. ‖

    Present address: Johnson & Johnson company, 3210 Merryfield Row, San Diego, CA 92121, USA.

  5. §

    Present address: Department of Biochemistry and Molecular Biology, Michigan State University, E. Lansing, MI 48824-1319, USA.

* (fax +1 607 254 2958; e-mail gj32@cornell.edu).

Publication History

  1. Issue published online: 7 AUG 2007
  2. Article first published online: 25 JUL 2007
  3. Received 23 January 2007; revised 20 March 2007; accepted 23 March 2007.

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Keywords:

  • benzoyloxyglucosinolate;
  • benzoyl-CoA ligase;
  • glucosinolate;
  • seed;
  • Arabidopsis;
  • mutant

Summary

Glucosinolates are secondary metabolites involved in pathogen and insect defense of cruciferous plants. Although seeds and vegetative tissue often have very different glucosinolate profiles, few genetic factors that determine seed glucosinolate accumulation have been identified. An HPLC-based screen of 5500 mutagenized Arabidopsis thaliana lines produced 33 glucosinolate mutants, of which 21 have seed-specific changes. Five of these mutant lines, representing three genetic loci, are compromised in the biosynthesis of benzoyloxyglucosinolates, which are only found in seeds and young seedlings of A. thaliana. Genetic mapping and analysis of T-DNA insertions in candidate genes identified BZO1 (At1g65880), which encodes an enzyme with benzoyl-CoA ligase activity, as being required for the accumulation of benzoyloxyglucosinolates. Long-chain aliphatic glucosinolates are elevated in bzo1 mutants, suggesting substrate competition for the common short-chain aliphatic glucosinolate precursors. Whereas bzo1 mutations have seed-specific effects on benzoyloxyglucosinolate accumulation, the relative abundance of 3-benzoyloxypropyl- and 4-benzoyloxybutylglucosinolates depends on the maternal genotype.

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