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Figure S1. Dwarfing and delayed flowering in AtGPS RNAi lines. Phenotype of plants transformed with AtGPS RNAi constructs. Individual RNAi lines (T1, top row) and control plants (bottom row) are shown.

Figure S2. Embryo-lethality in an AtGPS T-DNA insertion line. The T-DNA insertion in AtGPS of line GABI-Kat 097G02 (GenBank AL755431) was verified by PCR, hemizygous plants (gps-1) were selfed and the percentage of aborted embryos in siliques was counted. Wild-type controls were seggregated plants from the same seed-batch. The bar graph displays the average percentage of aborted embryos per silique and the standard error. 30 to 40 siliques were counted. On the right side an example of a silique with three aborted embryos is shown.

Figure S3. Expression of five plastidial AtGGPSs in AtGPS RNAi lines. Real-time Q-RT-PCR analysis of AtGGPS7,9,10,11,12 expression (At3g14550, At3g29430, At3g32040, At4g36810 and At4g38460, respectively) in dwarfed AtGPS RNAi lines. Expression levels were corrected for Actin expression and are displayed relative to expression in control plants (set to 100%). Standard errors are included.

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TPJ_3273_sm_figS1.pdf10156KSupporting info item
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TPJ_3273_sm_figS3.pdf74KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.