Present address: Chris C. N. van Schie, University of California San Diego, 9500 Gilman Drive #380, La Jolla, CA 92093-0380, USA.
Geranyl diphosphate synthase is required for biosynthesis of gibberellins
Article first published online: 18 SEP 2007
The Plant Journal
Volume 52, Issue 4, pages 752–762, November 2007
How to Cite
Van Schie, C. C. N., Ament, K., Schmidt, A., Lange, T., Haring, M. A. and Schuurink, R. C. (2007), Geranyl diphosphate synthase is required for biosynthesis of gibberellins. The Plant Journal, 52: 752–762. doi: 10.1111/j.1365-313X.2007.03273.x
- Issue published online: 18 SEP 2007
- Article first published online: 18 SEP 2007
- Received 9 April 2007; revised 16 July 2007; accepted 25 July 2007.
Figure S1. Dwarfing and delayed flowering in AtGPS RNAi lines. Phenotype of plants transformed with AtGPS RNAi constructs. Individual RNAi lines (T1, top row) and control plants (bottom row) are shown.
Figure S2. Embryo-lethality in an AtGPS T-DNA insertion line. The T-DNA insertion in AtGPS of line GABI-Kat 097G02 (GenBank AL755431) was verified by PCR, hemizygous plants (gps-1) were selfed and the percentage of aborted embryos in siliques was counted. Wild-type controls were seggregated plants from the same seed-batch. The bar graph displays the average percentage of aborted embryos per silique and the standard error. 30 to 40 siliques were counted. On the right side an example of a silique with three aborted embryos is shown.
Figure S3. Expression of five plastidial AtGGPSs in AtGPS RNAi lines. Real-time Q-RT-PCR analysis of AtGGPS7,9,10,11,12 expression (At3g14550, At3g29430, At3g32040, At4g36810 and At4g38460, respectively) in dwarfed AtGPS RNAi lines. Expression levels were corrected for Actin expression and are displayed relative to expression in control plants (set to 100%). Standard errors are included.
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