Evidence for a sorting endosome in Arabidopsis root cells
Article first published online: 4 OCT 2007
The Plant Journal
Volume 53, Issue 2, pages 237–247, January 2008
How to Cite
Jaillais, Y., Fobis-Loisy, I., Miège, C. and Gaude, T. (2008), Evidence for a sorting endosome in Arabidopsis root cells. The Plant Journal, 53: 237–247. doi: 10.1111/j.1365-313X.2007.03338.x
- Issue published online: 4 OCT 2007
- Article first published online: 4 OCT 2007
- Received 3 August 2007; revised 18 September 2007; accepted 20 September 2007.
- protein trafficking;
- pre-vacuolar compartment;
- root cells;
- sorting nexin;
- sorting endosome
In eukaryotic cells, the endocytic and secretory pathways are key players in several physiological processes. These pathways are largely inter-connected in animal and yeast cells through organelles named sorting endosomes. Sorting endosomes are multi-vesicular compartments that redirect proteins towards various destinations, such as the lysosomes or vacuoles for degradation, the trans-Golgi network for retrograde transport and the plasma membrane for recycling. In contrast, cross-talk between the endocytic and secretory pathways has not been clearly established in plants, especially in terms of cargo protein trafficking. Here we show by co-localization analyses that endosomes labelled with the AtSORTING NEXIN1 (AtSNX1) protein overlap with the pre-vacuolar compartment in Arabidopsis root cells. In addition, alteration of the routing functions of AtSNX1 endosomes by drug treatments leads to mis-routing of endocytic and secretory cargo proteins. Based on these results, we propose that the AtSNX1 endosomal compartment represents a sorting endosome in root cells, and that this specialized organelle is conserved throughout eukaryotes.