Data deposition footnote: Microarray data were uploaded in accordance with the MIAME standard to BASE (https://base.lcb.uu.se/) for worldwide public access.
RLM3, a TIR domain encoding gene involved in broad-range immunity of Arabidopsis to necrotrophic fungal pathogens
Article first published online: 4 APR 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
The Plant Journal
Volume 55, Issue 2, pages 188–200, July 2008
How to Cite
Staal, J., Kaliff, M., Dewaele, E., Persson, M. and Dixelius, C. (2008), RLM3, a TIR domain encoding gene involved in broad-range immunity of Arabidopsis to necrotrophic fungal pathogens. The Plant Journal, 55: 188–200. doi: 10.1111/j.1365-313X.2008.03503.x
- Issue published online: 8 JUL 2008
- Article first published online: 4 APR 2008
- Received 5 November 2007; revised 31 January 2008; accepted 29 February 2008.
- broad-range resistance;
- expression-level polymorphism (ELP);
- R gene;
- gene expression marker;
Here, we describe the rapid cloning of a plant gene, Leptosphaeria maculans 3 (RLM3Col), which encodes a putative Toll interleukin-1 receptor-nucleotide binding (TIR-NB) class protein, which is involved in defence against the fungal pathogen L. maculans and against three other necrotrophic fungi. We have, through microarray-based case control bulk segregant comparisons of transcriptomes in pools of Col-0 × An-1 progeny, identified the absence of a locus that causes susceptibility in An-1. The significance of this locus on chromosome 4 for L. maculans resistance was supported by PCR-based mapping, and denoted resistance to RLM3Col. Differential susceptible phenotypes in four independent T-DNA insertion lines support the hypothesis that At4g16990 is required for RLM3Col function. The mutants in RLM3Col also exhibited an enhanced susceptibility to Botrytis cinerea, Alternaria brassicicola and Alternaria brassicae. Complementations of An-1 and T-DNA mutants using overexpression of a short transcript lacking the NB-ARC domain, or a genomic clone, restored resistance to all necrotrophic fungi. The elevated expression of RLM3Col on B. cinerea-susceptible mutants further suggested convergence in signalling and gene regulation between defence against B. cinerea and L. maculans. In the case of L. maculans, RLM3Col is required for efficient callose deposition downstream of RLM1Col.